No abstract

Fructose, glucose, and sucrose in sugarcane molasses are determined simultaneously by high performance liquid chromatography (HPLC) using maltose as internal standard (IS) with refractive index detector (RID). The mixture of the diluted samples and IS was purified withSep-Pak C 18 solid-phase extraction (SPE) and filtered through a 0.22-μm membrane before injection. The results showed that the linear ranges for fructose, glucose and sucrose were Downloaded by [Baskent Universitesi] at 12:40 20 December 2014 ACCEPTED MANUSCRIPT ACCEPTED MANUSCRIPT 2 3.30-16.48 g/L, 1.80-9.02 g/L and 5.94-29.70 g/L with the squared correlation coefficients (R 2 ) being 0.9986, 0.9987 and 0.9955, respectively. The method is simple, quantified, and time-saving for determination of sugars in sugarcane molasses.

No abstract