Brucella species are equipped with a variety of well-organized immune evasion strategies to establish chronic infection, including the use of small non-coding RNAs (sRNAs). In this review, the involvement of sRNAs in regulation of virulence and intracellular survival of Brucella is discussed. Further it highlights the role of sRNAs in modulation of innate and adaptive immune response mechanism of Brucella, which will improve our understanding of brucellosis and development of effective therapeutic approaches to treat this infectious disease.