Harmful effects caused by aflatoxin (AF) directed researchers towards to find out new strategies for its control and detoxification increasing the safety of poultry feed. The aim of the present work was to study the protective role of date pits (Phoenix dactylifera) seeds against aflatoxicosis regarding carcass traits, biochemical function tests and histopathology of both liver and kidney in broiler chickens. 210 one-day old Arbor Acres broiler chicks were allotted into 7 equal groups as the first control (G1) supplemented by the basal diet, G2 had the basal diet with date pits supplementation 2%, G3 fed on the basal diet with date pits 4%, G4 was fed a basal diet containing 100µg aflatoxin/kg (100 ppb). G5 fed on a basal diet containing Hydrated Sodium Calcium Aluminum Silicates (HSCAS) 0.3% plus aflatoxin, (G6) fed a basal diet containing date pits 2% plus aflatoxin and finally G7 fed a basal diet containing date pits 4% plus aflatoxin. The aflatoxin supplemented to the broiler ration from first day to the end of experiment at 35 days. Aflatoxins supplementation significantly increased relative liver and small intestine weight, affect liver and kidney biochemical function tests and induced histopathological changes as fatty degeneration of hepatocytes, and interstitial nephritis with mononuclear cell infiltrations in both liver and kidney, respectively. However, addition of date pits (2% and 4%) and HSCAS (0.3%) to broiler's diet partially ameliorated these harmful effects of aflatoxins, indicating their protective effect against aflatoxicosis and this protection is dose-related. Addition of date palm seed (2% and 4%) gave a better results regarding carcass traits, biochemical parameters and histopathological examination of liver and kidney, finally concluding that date palm seed powder could be used as an effective feed additive to control aflatoxicosis in poultry with avoiding harmful effect of chemical mycotoxin binders (HSCAS).