The aim of this study is to extract the important antioxidants components of flaxseed in different ways. The Determination of total phenol content, antioxidant activity and percentage of linoleic acid inhibition by DPPH for the components (Lignan, oil, 80 and 100% ethanolic extract and the deionized water extract). The components of flaxseed oil were identified using GC/MS. The efficiency of the mixture (80% ethanolic extract and oil)  was determined by inhibiting the process of oxidation of linoleic acid during storage periods (0, 7, 14 and 21 days) at laboratory temperature, and the following results were obtained:  The yield of lignan, oil, ethanol extract (80 and 100)% and deionized  water were (0.12,40.52,10.9,9.6 and 13 )g.100g-1 seed respectively, while the total phenolic content of the above components was (700,1165,3315,2098 and 483)mgGAE.100g-1,respectively. Flaxseed oil gave the highest antioxidant activity 79.3% with an inhibition percentage 73.19%, compared with ethanolic extract, lignan and water extract. The diagnosis of GC/MS flaxseed oil showed that  the oil was contained in compounds with antioxidant activity, including mono, di and tri-terpens such as Copaene, Monoterpene, ?- Sesquiphellandrene, Squalene, diethyl phthalate ,?-Sitosterol and Hexadecanoic acid, as well as, the presence of ascorbic acid and gamma tocopherol with good concentrations (8.68 and 2.62)% respectively. The mixture of 80% ethanol and oil extract showed an effect in decreasing of peroxide values with an increase of concentration of the added mixture (0.1, 0.4 and 0.10 mg.mg-1). The maximum reduction of the peroxide value at the concentration was 0.6 mg. mg-1, compared with the standard sample during the different durations of storage.

Fructooligosaccharide is used widely in many foods and pharmaceutical industries and produced by using different ways such as extracting it from plants or producing it by using plants and microorganisms’ enzymes. In a previous study, we extracted Fructosyltransferase (Ftase) enzyme from pineapple residue and produced FOS. In this study, we measured the antagonistic activity of two synbiotics, the first synbiotic containing Lactobacillus acidophilus and the produced FOS, the second synbiotic containing Lactobacillus acidophilus and standard FOS, against pathogenic bacteria (P. aeruginosa, E. coli, S. aureus and B cereus). The results showed that the antagonistic activity of both synbiotic types was very close, as there were no significant differences between them except in the antagonistic activity against S. aureus, there was a significant difference between the synbiotic containing the standard FOS, which was the highest in its antagonistic activity compared to the synbiotic containing the produced FOS in this study. The activity of the fructooligosaccharide (FOS) extracted from pineapple residue was evident in enhancing the activity of the probiotic bacteria (L. acidophilus), which had a major role in the production of acids and compounds that inhibited the pathogenic bacteria. The diameters of inhibition areas in the current study ranged between 19.33-28 mm, and E. coli was more susceptible to inhibition, followed by S. aureus, P. aeruginosa, and B. cereus, respectively.

Conjugated Linoleic Acid (CLA) has attracted the attention of many researchers, especially that of microbial origin due to its biological importance to the consumer. The current study aims to extract LA Isomerase enzyme from Lactobacillus paracasei bacteria from milk and to use the enzyme in the production of CLA. Selective media, including MRS and MRS-Dagatose, were used in isolating local strains. The selected bacterial isolates were tested for their ability to produce LA-Isomerase enzyme. The isolate with high enzymatic activity was selected. After extraction and partial purification of the enzyme, the optimal conditions for the production of conjugated fatty acid were studied, and the reaction products were diagnosed using GC-MS technology. It was found that 11 isolates have the ability to produce CLA at different concentrations, H1 isolate showed the highest production of conjugated fatty acid at a concentration of 120.45 g.ml-1, this isolate was selected as the source for enzyme extraction. The enzymatic activity of the crude extract and partially purified with ammonium sulfate was estimated using color methods at wavelength of 233 nm. The effect of the optimum conditions (pH, temperature, linoleic acid concentration and enzyme concentration) on the CLA product was studied using the partially purified LA Isomerase enzyme, the optimum conditions for production were 6.5, 45 °C, 100 μg.ml-1 and 0.7 ml, respectively. The GC-MS technique showed the presence of a number of reaction products that are isomers of conjugated linoleic acid (C9T11, T9T12, T10C12) with different concentrations.