Salt stress is considered as one of the most common abiotic stresses reducing the productivity and fruit quality of crop plants. The present study was carried out to assess the salt tolerance among 15 local squash (Cucurbita maxima Duchesne) landraces. Different salt (NaCl) concentrations of 0, 100, 200 and 300 mM were selected in order to evaluate the response of the study germplasm to salt stress based on 12 agronomic parameters and 3 biochemical traits, proline, malondialdehyde (MDA) and chlorophylls. A varied effect of the salt stress level was observed among the studied landraces based on germination potential, as well as on growth and biochemical parameters at seedling stage. Results showed that all landraces were drastically affected at high stress level with a significant variation in their stress response, indicating the existence of considerable genetic variability. Landraces “746” and “747” were the best performing cultivars across stress levels, whereas “1007”, “1008” and “1009” were the most negatively affected. Based on the tested landrace performance, four landraceswere selected and further evaluated at biochemical level, focusing on the determination of compounds that play a key role in the ability to withstand salt stress. The mean MDA content across landraces was generally increased in stressed plants, as compared to the control treatment; the increase was attributed to a peak in MDA content at specific stress levels. In particular, “746” and “1007” showed the maximum content at 100 mM NaCl, while in landrace “751”, MDA content reached its peak at 300 mM NaCl. In addition, the response of most landraces to salt stress involved an increase in free proline content, with the exception of “746”, with the maximum content being observed either at 200 mM (“748” and “751” landraces) or at 300 mM NaCl, where only “747” expressed the highest content. These findings can be extrapolated into efforts to develop more salt-tolerant squash landraces and exhaust the possibilities of using saline water or soils under changing climate conditions.
Trace elements in the environmental media contribute to toxicities of different types. Their presence in the arable pedosphere is a human-health risk factor. This study focused on Vicia faba represented by two Tunisian varieties of bean (Mamdouh) and faba bean (Badii). The objective was to analyze the effects of lead, copper and cadmium on their growth, chlorophyll-content and carotenoids-content, as well as the bioaccumulation and translocation factor, at different stages of growth. For each metal, the concentrations the plants were subjected to were 6, 0.3 and 0.03 mg/L of the metal in the compound for lead nitrate, copper nitrate and cadmium acetate, respectively. The analysis was carried out using an atomic absorption spectrophotometer (ICP-MS), encompassing all the parts of the plant. The authors detected a perceptible decrease in the fresh weight of roots and shoots, as well as a drop in the chlorophyll and carotenoid, for all the three heavy metals. Cadmium turned out to be the most toxic of the three metals and copper (which is incidentally an essential micronutrient for plant growth) the least. As far as the bioaccumulation factor was concerned, bean and faba bean exhibited different behaviours, both with regard to the growth stages and the heavy metal absorbed. During the vegetative growth stage, both were accumulators of all the three heavy metals (a translocation factor less than unity). However, in the flowering stage, faba bean was a hyper-accumulator of copper (TF > 1); while the bean plants accumulated a lot of lead in the pods-stage (TF > 1). It is worthwhile to pose new research questions and try to answer them in this study, if legumes are accumulator or hyper accumulator plants in which stage and in where organ accumulate more HMs.
In this work, we studied agro-morphological parameters that could be used to define variaton among early, mid-early, mid-late and late potato cultivars, cropped in the main season by quantitaive and qualitative parameters (characters of mother tubers, vegetation cover, height of plants, number of leaves, number of true leaves, number of stems, number of stolons, lenght of first stolon, median diameter of first stolon, caliber of first tuber, final number of tubers per plant and final yield per plant). For the main agro-morphological characters, there were a great variation compared to the control Spunta. Challenger and Fabula showed the heighest caliber of mother tubers (49.16 mm), while heighest weight of seed tubers was determined on cv. Alegria (125.7 g). Among other characters, challenger produced the largest leaf area (9940.3 mm²). Panamera cultivar has considerable plant height (13.77 cm), the heighest number of leaves per plant (63.33) and also the heighest number of true leaves (48.48). Moreover, Panamera has the largest lenght of first stolon with 8.66 cm, (6.16 cm for Spunta). It has also the largest value of median diameter of first stolon with 2.17 mm and followed by Mondial (2.23 mm), Bellini (1.63 mm) and Liseta (2.01 mm). The difference among the cultivars in terms of median diameter of first stolon was statistically significant (P≤0.05). Liseta has the largest value of caliber of first tuber (37.4 mm), followed by Bellini (37.71 mm), while, Spunta has presented the lowest measures (13.36 mm). For final yield per plant, Bellini outyielded other cultivars with 0.96 kg per plant.
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