No abstract

SummaryRestriction fragment length polymorphisms were studied among the Japanese population using 12 polymorphic DNA probes on the q24-q28 region of X chromosome. The frequency distribution for probes p22-33, p482.6a, p43-15, 52A, pPM101, cX33.2 and cpx234, was the same as that for Caucasians, and that for probes 4D-8 and Stl4-1 (MspI) was slightly different (p<0.05). However, it was quite different (p<0.01) for probes p114.12, Stl4-1 (TaqI), 36B-2 and MN12. Probe p114.12 showed no HindIII polymorphism for the Japanese people. On the contrary, probe MN12, which has a low PIC value (0.15) for Caucasians, was found to be useful for Japanese (PIC value=0.50). These results suggest that 7 DNA probes (p482.6a, p43-15, 52A, Stl4-1, p114.12 (BclI), 36B-2 and MN12) are useful (PIC > 0.42) for linkage analysis of X-linked disease in Japan.

SummaryRestriction fragment length polymorphisms were studied among the Japanese using 13 polymorphic DNA probes on the X chromosome. For 6 probes (pPA4B, cpX203, p58-1, pHPGK-7e, cpX289 and 7b) the allelic frequencies were the same as those for Caucasians, but they were quite different (p < 0.01) for 4 probes (dic56, pOTC (MspI), pTAK8B and pXG-16 (HindlII)). No polymorphisms were observed for 4 probes (pG95M-7dIII/RI (n (chromosome number studied)--54), pXG-16 (TaqI) (n=50), p8 (n=108), and pXG-17 (n=76). These results suggest that not a small number of DNA probes currently available are useless for linkage analysis in Japan.