Aims/HypothesisSeveral studies have shown that adiponectin can lower blood glucose in diabetic mice. The aim of this study was to establish an effective adiponectin production process and to evaluate the anti-diabetic potential of the different adiponectin forms in diabetic mice and sand rats.MethodsHuman high molecular weight, mouse low molecular weight and mouse plus human globular adiponectin forms were expressed and purified from mammalian cells or yeast. The purified protein was administered at 10–30 mg/kg i.p. b.i.d. to diabetic db/db mice for 2 weeks. Furthermore, high molecular weight human and globular mouse adiponectin batches were administered at 5–15 mg/kg i.p. b.i.d. to diabetic sand rats for 12 days.ResultsSurprisingly, none of our batches had any effect on blood glucose, HbA1c, plasma lipids or body weight in diabetic db/db mice or sand rats. In vitro biological, biochemical and biophysical data suggest that the protein was correctly folded and biologically active.Conclusions/InterpretationRecombinant adiponectin is ineffective at lowering blood glucose in diabetic db/db mice or sand rats.

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Based on the whole development plan of vocation tourism in Badong country, the development status and problems were analyzed for Yesanguan ecological vacation tourism in western Hubei province. The strategic orientation, strategic targets and measures of Yesanguan ecological vacation tourism are proposed. Through resource integration, administrative merge and unified plan, the new ecotourism products of southeast Badong are formed, and the Yesanguan tourist hub and Lucongpo alpine leisure international club would be built.