Aim Epithelial-mesenchymal transition (EMT) of retinal pigment epithelium (RPE) cells is the key of the development of diabetic retinopathy (DR), and lncRNA NEAT1 could accelerate EMT in diabetic nephropathy. Meanwhile, as a diabetes susceptibility gene, whether sex-determining region Y-related (SRY) high-mobility group box 4 (SOX4) has relationship with lncRNA NEAT1 in DR remains unclear. Methods Firstly, NEAT1, SOX4 and miR-204 were evaluated by qRT-PCR (quantitative reverse-transcriptase PCR) under high glucose condition. Then, cell viability, proliferation, migration and invasion were respectively detected by MTT, BrdU staining, wound healing and transwell assay after NEAT1 knockdown or miR-204 overexpression. Also, the EMT-related proteins were examined by western blot and cell immunofluorescence assay. In order to confirm the relationship between miR-204 and NEAT1 or SOX4, dual luciferase reporter gene assay was conducted. At the same time, the protein levels of SOX4 and EMT-related proteins were investigated by immunohistochemistry in vivo. Results High glucose upregulated NEAT1 and SOX4 and downregulated miR-204 in ARPE19 cells. NEAT1 knockdown or miR-204 overexpression inhibited the proliferation and EMT progression of ARPE19 cells induced by high glucose. NEAT1 was identified as a molecular sponge of miR-204 to increase the level of SOX4. The effect of NEAT1 knockdown on the progression of EMT under high glucose condition in ARPE19 cells could be reversed by miR-204 inhibitor. Also, NEAT1 knockdown inhibited retinal EMT in diabetic mice. Conclusion NEAT1 regulated the development of EMT in DR through miR-204/SOX4 pathway, which could provide reference for clinical prevention and treatment.

Aiming at the shortcomings of traditional research method of materials mechanics performance of concrete, this paper presents a new method to test the mechanical properties of concrete material layer body--the relative method. It focuses on the use of relative principle, testing dynamic elastic modulus of concrete damage layer subjected to single salt freezing cyclic, and draw a gradient damage curve of concrete, and then study the damage process of the concrete under the effect of salt solution and the freeze-thaw cycle synergetic factors. The method provides a new way to test body layer mechanical performance of concrete material which the traditional methods can not be directly solved.

Purpose. The goal of this study was to see if electroacupuncture was effective and safe in the management of cerebral infarction. PubMed, Embase, Cochrane Library, and Web of Science were used to conduct a comprehensive literature survey. Methods. Basic features of 7 studies were identified using the searching strategy. The investigation was found in PubMed, Embase, and Web of Science, with the most recent search being in March 2022. “Electroacupuncture,” “cerebral infarction,” and their permutations were among the MeSH terms and free words used. As literature, two reviewers independently used a standardized form to gather pertinent data from qualifying research. Results. 157 literatures were identified and evaluated. Electroacupuncture improved the BI score in individuals with cerebral infarction ( mean   difference = 0.10 , 95 percent CI: 0.00-0.20, p = 0.04 ). Electroacupuncture enhanced BI score in individuals with cerebral infarction ( mean   difference = 0.10 , 95 percent CI: 0.00-0.20, p = 0.04 ). The effects of electroacupuncture increased Fugl-Meyer index in patients with cerebral infarction ( mean   difference = 25.92 , 95% CI: 25.28-26.56, p < 0.00001 ). Electroacupuncture effects decreased CSS in patients with cerebral infarction in the experiment group ( mean   difference = − 2.10 , 95% CI: -2.53–-1.67, p < 0.0001 ). Electroacupuncture also reduced CSS individuals with cerebral infarction in the control group; however, there was no statistically significant ( risk   difference = 0.06 , 95 percent CI: 0.02-0.13, p = 0.12 ). Conclusion. This study demonstrated that electroacupuncture helped decreased CSS in patients with cerebral infarction.

Basing on the experimental data collected, the mechanical model of reinforced concrete beams’ gradual failure has been set up, to test differ reinforced components with loads and study the process of split. At last the influence of reinforcement’s number on components’ intensity and failure process are analyzed by using MFPA2D (Material Failure Process Analysis) software, started with the analysis of formula cracks after experiments. The results showed that the number of reinforcement is not the more the better. When ultra-reinforced beam appears, its mechanical capacity enhances but the bars won’t do their best, furthermore, it is uneconomical. Accordingly, less-reinforced beam should also be avoided for safe. Everything possible should be made to avoid ultra-reinforced beam and less-reinforced beam in engineering. By all means, this conclusion has practical significance during our practice.

The fracture behavior of brittle materials under biaxial plane stress has been investigated by means of numerical simulation method with software MFPA2D (Material Failure Process Analysis). The aims of this study are to clarify the fracture dependence of brittle material on biaxial plane stress state. The observation of crack initiation and fracture behavior reveals that the biaxial stresses have strong influence on the fracture properties of glass. Thus, the fracture criterion by the stress intensity factor was questioned for the biaxial plane stress issues. It is confirmed that the tensile stress parallel to the crack plane is an important factor affecting crack arrest, while the compressive stress parallel to the crack plane contributes to crack opening.