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Recently, it has been demonstrated that thermoviscous flows can be used for a range of fine micromanipulations, such as moving the cytoplasm of cells and developing embryos, intracellular rheology, and femtonewton-range force measurements. These flows, also known as focused-light-induced cytoplasmic streaming (FLUCS), are induced by mid-infrared laser scanning of a temperature spot through the sample. However, localized laser scanning can inflict temperature perturbations of several Kelvins on the sample, potentially eliciting unspecific biological responses. In this study, we demonstrate how exploiting symmetry relations during laser scanning effectively disentangles laser heating and flow induction. We introduce flow-neutral scan sequences that use dynamic photothermal stimuli and spatiotemporal symmetry relations of scanning bridging up to three distinct time scales. We leverage further insights from a recently published analytical model of flow fields to present quasi-homogenous temperature distributions that leave flow lines and their local and directed character largely invariant. We present practical, intuitive solutions through predesigned sets of scan lines with near isothermal distributions and demonstrate that they are sufficient to generate and control flows in Caenorhabditis elegans embryos on a magnitude well in excess of endogenous flow velocities. Our results enable the separation of two previously tightly linked classes of physical stimuli, introduce a new, even less invasive standard for performing FLUCS perturbations, and pave the way for new unexplored avenues in the fields of soft matter and biomedicine. Graphical Abstract

Recent experiments in cell biology have probed the impact of artificially-induced intracellular flows in the spatiotemporal organisation of cells and organisms. In these experiments, mild dynamical heating (a few kelvins) via focused infrared light from a laser leads to long-range, thermoviscous flows of the cytoplasm inside a cell. To extend future use of this method in cell biology, popularised as focused-light-induced cytoplasmic streaming (FLUCS), new quantitative models are needed to link the external light forcing to the produced flows and transport. Here, we present a fully analytical, theoretical model describing the fluid flow induced by the dynamical laser stimulus at all length scales (both near the scan path of the laser beam and in the far field) in two-dimensional confinement. We model the effect of the focused light as a small, local temperature change in the fluid, which causes a small change in both the density and the viscosity of the fluid locally. In turn, this results in a locally compressible fluid flow. We analytically solve for the instantaneous flow field induced by the translation of a heat spot of arbitrary time-dependent amplitude along a scan path of arbitrary length. We show that the leading-order instantaneous flow field results from the thermal expansion of the fluid and is independent of the thermal viscosity coefficient. This leadingorder velocity field is proportional to the thermal expansion coefficient and the magnitude of the temperature perturbation, with far-field behaviour typically dominated by a source or sink flow and proportional to the rate of change of the heat-spot amplitude. In contrast, and in agreement with experimental measurements, the net displacement of a material point due to a full scan of the heat spot is quadratic in the heat-spot amplitude, as it results from the interplay of thermal expansion and thermal viscosity changes. The corresponding average velocity of material points over a scan is a hydrodynamic source dipole in the far field, with direction dependent on the relative importance of thermal expansion and thermal viscosity changes. Our quantitative findings show excellent agreement with recent experimental results and will enable the design of new controlled experiments to establish the physiological role of physical transport processes inside cells.

Taking the wind turbine tower as the research object, based on the finite element software, a simplified beam-shell hybrid element model was first established; through the simulation, the phase difference between the loose position and the unloose position was compared to verify the feasibility of the phase difference detection method; Secondly, the influence of the number of loose bolts, the position of loosening, and the magnitude of the wind force on the phase of the flange bolt connection structure and the response characteristics of the system are analyzed. The research results show that the number of loose bolts, the position of loosening, and the magnitude of the wind have certain effects on the phase difference and response characteristics of the flange. With the increase in the number of loose bolts, the connection stiffness of the bolt connection continues to decrease. The linear characteristic is enhanced; the closer the loosening is to the excitation force loading position, the greater the detected phase difference; as the wind increases, the phase of the upper flange of the tower changes, and the phase of the lower flange remains unchanged, and the wind is on the flange The disc connection strength has little effect.