Microalgae is a promising candidate for reducing greenhouse gas and producing renewable biofuels. For microalgae biofilm cultivation, a strong adhesion ability of microalgae cells onto the surface is a prerequisite to resist the fluid shear stress, while strong adhesion is not of benefit to the biofilm harvesting process. To solve this dilemma, a thermoresponsive surface (TMRS) with lower critical solution temperature of 33 °C was made by grafting N-isopropylacrylamide onto a silicate glass slide. The wettability of the TMRS changed from hydrophilic (contact angle of 59.4°) to hydrophobic (contact angle of 91.6°) when the temperature rose from 15 to 35 °C, resulting in the increase of adhesion energy of the TMRS to Chlorella vulgaris cells by 135.6%. The experiments showed that the cells were more likely to attach onto the TMRS at the higher temperature of 35 °C owing to the surface microstructures generated by the hydrogel layer shrinkage, which is similar in size to the microalgae cells. And the cell coverage rate on TMRS increased by 32% compared to the original glass surface. Conversely, the cells separate easily from the TMRS at a lower temperature of 15 °C, and the cell adhesion density was reduced by 19% due to hydrogel layer swelling to a relatively flat surface.

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Microalgal biofilm, a stable community of many algal cells attached to a solid substrate, plays a significant role in the efficient accumulation of renewable energy feedstocks, wastewater treatment, and carbon reduction. The adhesion tendency of microalgal cells on solid substrates is the basis for controlling the formation and development of microalgal biofilm. To promote the adhesion of microalgal cells on solid substrates, it is necessary to clarify which surface properties have to be changed in the most critical factors affecting the adhesion. However, there have been few systematic discussions on what surface properties influence the adhesion tendency of algal cells on solid substrates. In this study, the essential principle of microalgal cell adhesion onto solid substrates was explored from the perspective of the interaction energy between microalgal cells and solid substrates. The influence of surface properties between microalgal cells and solid substrates on interaction energies was discussed via extended Derjaguin–Landau–Verwey–Overbeek (eDLVO) theory and a sensitivity analysis. The results showed that surface properties, including surface potential (ξ) and surface free energy components, significantly affect the adhesion tendency of microalgal cells on different solid substrates. When the solid surface possesses positive charges (ξ > 0), reducing ξ or the electron donor components of the solid substrate (γs –) is an effective measure to promote microalgal cell adhesion onto the solid substrate. When the solid surface possesses negative charges (ξ < 0), an increase in either γs – or the absolute value of ξ should be avoided in the process of microalgae adhesion. Overall, this research provides a direction for the selection of solid substrates and a direction for surface modification to facilitate the adhesion tendency of microalgal cells on solid substrates under different scenarios.