Acetate was inhibitory to the growth of early induced E. cofi cells and their expression of fusion protein, transforming growth factor-a-Pseudomonas exotoxin 40 (TGFa-PE40), but the inhibitory level was strain dependent For E. coli JM109 (pTAC-TGF57-PESO). 2 g/L of added acetate (3 g/L of total acetate in the medium) decreased TGFa-PEA0 production by 38.0%. Acetate was less inhibitory to E. coli RRl. and RR1 was not affected by adding 2 B/L of acetate. However, 5 g/L of added acetate (6.7 g,L of total acetate in the medium) decreased TGFa-PE40 production by 21.2%. These results indicate that higher acetate concentration was associated with inhibition of TGFa-PE40 expression of E. coli JM109 during hue induction.

Traditional methods for single-nucleotide variants based on the amplification and the fluorescent signals require expensive reagents and cumbersome instruments, and they are time-consuming for each trial. Here, a porous anodised...

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