Objective: The proposal of the studying was to investigate the protective effect of Limb ischemic precondition or Dezocine pre-treatment in acute stress-related gastric mucosal injury.Methods: Forty-five Sprague Dawley (SD) male rats at 2-3 month were randomly divided into three groups: controlled group (Group C, n=15), Ischemic precondition group (Group T, n=15): rats in this group received 5 cycles of ischemic precondition of 5-5 min in their left legs; Dezocine pre-treatment group (Group D, n=15): Rats in this group received Dezocine subcutaneous injection at a dosage of 10µg/g. Rats were beheaded after 6h of cold water immerse, and stomach was taken out for Gastric injury index (GI), HE stains, and measurement of MDA, SOD, MPA. Result:(1) There were significant lower GI scores and less Inflammatory response in gastric (fewer Neutrophils) in Group T and Group D when compared to Group C, (p<0.05, CI=95%);(2) There levers of MDA and MPA were significantly lower in Group T and Group D when compared to That of Group C,(p<0.05, CI=95%) and levers of SOD in Group T and Group D were significantly higher than that of Group C, (p<0.05, CI=95%);(3) Lever of SOD in Group T was higher than that in Group D,(P=0.048, CI=95%). Conclusion:Both Limb ischemic preconditioning and Dezocine pre-treatment will reduce acute gastric mucosal injury by inhibiting the inflammatory response and cleaning oxygen radical.