Two new compounds, an abscisic acid-type sesquiterpene (1), and one asterric acid derivative (2), together with three known compounds (3-5) were isolation from mangrove endophytic fungus Pleosporales sp. SK7. The structures of these metabolites were determined by NMR, X-ray crystal diffraction, CD and HR-ESI-MS. All compounds were tested for their antibacterial, antioxidant and cytotoxic activities, among these compounds, 5 showed cytotoxicity against MDA-MB-435 cell with an IC 50 of 25.96±0.32 μM.