In order to reduce the shock and vibration caused by torque disturbance of the gearbox in vehicles equipped with automatic transmission in the process of in situ shift, a novel semi-active hydraulic damping strut is introduced in the powertrain mounting system. The dynamic response evaluation indexes of vehicle in situ shift are put forward, and a 13-degree of freedom vehicle dynamic model including the semi-active hydraulic damping strut is established. The optimized dynamic characteristic parameters are acquired according to the principle of sharing force and the 13-degree of freedom vehicle dynamic model. The dynamic response evaluation indexes with and without the semi-active hydraulic damping strut are calculated using the 13-degree of freedom vehicle dynamic model in the process of in situ shift, and the calculation results show that the vibration of a vehicle can be reduced by the introduction of a semi-active hydraulic damping strut. Experiments are carried out to analyze the vibration response of the vehicle with and without a semi-active hydraulic damping strut, and the results show that the shock and vibration of the vehicle are reduced by introducing the semi-active hydraulic damping strut. The theoretical calculation values of active-side acceleration of the engine mount and torque strut are consistent with the experimental values, which show that the 13-degree of freedom vehicle dynamic model is reasonable.