Lung carcinoma is the most common type of malignant tumors globally, and its molecular mechanisms remained unclear. With the aim to investigate the effects of microRNA (miR)‐377‐5p on the cell development, invasion, metastasis, and cycle of lung carcinoma, this study was performed. We evaluated miR‐377‐5p expression levels in lung cancer tissues and cell models. Cell viability, proliferation, migration, invasion abilities, and cell cycle distribution were measured using 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide, crystal violet, transwell, and flow cytometry assay. Furthermore, expression levels of protein kinase B α subunit (AKT1) and proteins related to cell cycle and epithelial‐mesenchymal transition (EMT) were assessed using Western blot analysis and quantitative real‐time polymerase chain reaction. These results suggested that miR‐377‐5p was downregulated in vivo and in cell models, and miR‐377‐5p overexpression inhibited cell viability, proliferation, migration, invasion, and induced cell‐cycle arrest. In addition, as a target of miR‐377‐5p, AKT1 alleviated the decreases of cell viability, proliferation, migration, invasion, the S‐phase cells, the expression of cyclin D1, fibronectin, and vimentin, as well as the increases of the G0/G1‐phase cells, the expression of Foxo1, p27 kip1, p21 Cip1 and E‐cadherin when miR‐377‐5p overexpressed. In conclusion, miR‐377‐5p inhibited cell development and regulated cell cycle distribution and EMT by targeting AKT1, which provided a theoretical basis for further study of lung carcinoma therapeutics.

The application of gradient elution in high performance liquid chromatography brings great convenience to separation of organic compounds. It has now become an important part of high performance liquid chromatography. How to achieve gradient elution is the key problem in separation of organic compounds. This paper discusses Dual-pump Gradient System which is used to achieve gradient elution and improve separation efficiency.