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VC-co-VAc-OH material was used to prepare flat-sheet membranes on the non-woven fabrics via phase inversion method. And the membrane preparation conditions（polymer content, the content of additive, environmental temperature, relative humidity, evaporation time, coagulation temperature, coagulation time, immersing time of non-woven fabrics in solvent, and exposure time of non-woven fabrics in air）were discussed systemically by orthogonal method. Finally, the membrane prepared under the optimum condition showe higher pure water flux (287.9 ml•cm-2•h-1) and good retention of Bovine serum albumin (BSA) (87.4 %).