Though the greenhouse whitefly, Trialeurodes vaporariorum (Westwood) (Hemiptera: Aleyrodidae) was introduced into China more than 60 years ago, the genetic diversity and structure of this exotic insect pest and virus vector have not been studied. To investigate the population genetic characteristics of this invasive species and to identify potential invasion routes, the genetic diversity and population structure of 17 collections of T. vaporariorum from nine provinces in China were analyzed using seven microsatellite loci. The results of the analyses indicated that the genetic diversity for the populations examined from the four provinces: Jilin, Ningxia, Guizhou and Qinghai, was lower than the genetic diversity of populations from the five provinces: Yunnan, Shandong, Shanxi, Liaoning, and Gansu. The T. vaporariorum populations analyzed in this study grouped as two distinct genetic clusters based on the analysis using STRUCTURE, whereas, 8 clusters were identified based on the BAPS analysis. Of the 136 genetic distance (Fst) values, 128 (94%) were associated with a significant exact test. There was a significant relationship between Fst and geographical distance. These results demonstrate that populations of T. vaporariorum in China exhibit significant genetic differentiation, indicating the likelihood that multiple introductions of T. vaporariorum into China have occurred. Also, the populations collected from the provinces of Jilin, Ningxia, Guizhou and Qinghai appear to represent secondary introductions originating from other Chinese provinces.

In order to visualize pronunciation teaching and explore the law of articulation evolution in TCFL (Teaching Chinese as a Foreign Language), we proposed an approach to design a physiology Mandarin speech corpus for the international preparatory students in China. Preparatory students are usually beginners to learn Mandarin, so they are selected as testers to gain the most real law of Mandarin articulation evolution. The corpus was different from an ordinary speech corpus. It consisted of not only text information (such as age, gender, nationality, mother language, Mandarin learning experience, etc.) and audio information, but also the physiological data (such as speech organs’ moving morphology). When the corpus was constructed, data were gathered by a supersonic collecting system because of its safety and low cost. In the initial experiment, 21 initial consonants, 36 simple or compound vowels and tones were tested. The results showed the corpus was significant in Mandarin teaching.