Artificial selection has been practiced for centuries to shape the properties of individual organisms, providing Darwin with a powerful argument for his theory of natural selection. We show that the properties of whole ecosystems can also be shaped by artificial selection procedures. Ecosystems initiated in the laboratory vary phenotypically and a proportion of the variation is heritable, despite the fact that the ecosystems initially are composed of thousands of species and millions of individuals. Artificial ecosystem selection can be used for practical purposes, illustrates an important role for complex interactions in evolution, and challenges a widespread belief that selection is most effective at lower levels of the biological hierarchy.

Mycorrhizae regulate elemental and energy flows in terrestrial ecosystems. We understand much of how mycorrhizae work, but integrating all possible mechanisms into a whole has proven elusive. Multiple evolutionary events and the long evolutionary history mean that different plants and fungi bring independent characteristics to the symbiosis. This variety results in extensive physiological variation. How do we integrate functional responses with diversity to understand the role of mycorrhizae in ecosystems? We review ecophysiological mechanisms of mycorrhizae and organize these into functional groups. Species-area relationships are not curvilinear, but resemble the "broken stick" model. We coupled functional groups with a metacommunity analysis to show how complex behavior can be generated using a simple matrix model of resource exchange. This approach provides insights into how we might integrate diversity and function across landscapes.

Gastric digestion of walleye, Stizostedion vitreum vitreum, and sauger, Stizostedion canadense, was measured by pumping stomachs at various intervals after experimental meals had been force-fed or voluntarily consumed. No significant differences in digestion rates were found between species or among walleye taken from three lakes. Repeated use of fish in digestion experiments failed to influence results. Gastric digestion rate increased with fish size, but was depressed by increased meal size, food particle size, and by force-feeding experimental meals.Food consumption rate and feeding chronology of the strong 1966 year-class of Lake of the Woods walleye were determined by applying results of laboratory digestion studies to stomach samples. An estimating technique that takes into account the effects of factors shown to influence digestion in the laboratory was developed and used to make calculations for the field population. Consumption averaged 1% of body weight during June, 2% during July, and 3% during August and September. Average temperatures were similar during June and September. Greater consumption during September resulted from higher food availability. Feeding was greatest during night and early morning hours in all months except June when it was continuous. Conversion efficiency of the 1966 year-class was described from estimates of growth and consumption rates for June through September and approximated 20% during each month.

We present a method for selecting entire microbial ecosystems for bioremediation and other practical purposes. A population of ecosystems is established in the laboratory, each ecosystem is measured for a desired property (in our case, degradation of the environmental pollutant 3-chloroaniline), and the best ecosystems are used as 'parents' to inoculate a new generation of 'offspring' ecosystems. Over many generations of variation and selection, the ecosystems become increasingly well adapted to produce the desired property. The procedure is similar to standard artificial selection experiments except that whole ecosystems, rather than single individuals, are the units of selection. The procedure can also be understood in terms of complex system theory as a way of searching a vast combinatorial space (many thousands of microbial species and many thousands of genes within species) for combinations that are especially good at producing the desired property. Ecosystem-level selection can be performed without any specific knowledge of the species that comprise the ecosystems and can select ensembles of species that would be difficult to discover with more reductionistic methods. Once a 'designer ecosystem' has been created by ecosystem-level selection, reductionistic methods can be used to identify the component species and to discover how they interact to produce the desired effect.

SynopsisIn experiments lakes 223 (L223) and 302 South (L302S) in the Experimental Lakes Area in north-western Ontario, and Little Rock Lake (LRL) in northern Wisconsin, were progressively acidified with sulphuric acid from original pH values of 6.1–6.8 to 4.7–5.1. Although the lakes were at different locations with different physical settings and assemblages of plants and animals including fish, there were remarkable similarities in their responses, particularly in regard to biogeochemical processes and effects on biota at lower trophic levels.All three lakes generated an important part of their buffering capacity internally b\ the reduction of sulphate, and to a lesser extent by the reduction of nitrate. Alkalinity production increased as concentrations of biologically-active strong acid anions increased. Models relating the residence times of sulphate and nitrate to water renewal, or first-order kinetics, effectively predicted events.Acidification disrupted nitrogen cycling in all three lakes. Nitrification was inhibited in L223 and L302S, while in LRL, nitrogen fixation was greatly decreased at low pH.The phytoplankton communities of all three lakes were originally dominated by chrysophyceans and cryptophyceans. However acidification changed the dominant species and decreased diversity. Acidification tended to increase phytoplankton production and standing crop slightly, probably because light penetration was increased.Littoral zones of all three lakes became increasingly dominated by a few species of filamentous green algae, which created nuisance blooms by pH 5.6. Mats or clouds of algae changed the entire character of the littoral zone.Acidification of L223 and L302S caused the loss of several species of large benthic crustaceans as pH changed from 6 to 5.6. Large, acid-sensitive littoral crustaceans were absent from LRL before acidification, probably because the lake was already too acidic.As acidity increased, the dominance of cladocerans within zooplankton communities increased. Daphnia catawba appeared at pH values near 5.6 and became more abundant at lower pHs as the lakes were acidified. Its appearance coincided with a decline in other Daphnia species: another cladoceran, Bosmina longirostris, increased in the experimentally-acidified lakes as did Keratella taurocephala: they became the dominant rotifers. Several sensitive zooplankton species declined or disappeared as the lakes were acidified, most notably Daphnia galeata mendotae, Epischura lacustris, Diaptomus sicilis and Keratella cochlearis.The responses of different fish varied; they appeared to depend on the sensitivity of key organisms in the food chain. The ability of key fish species to reproduce was impaired as early as pH 5.8; their reproduction, except for yellow perch in LRL, had ceased at pH 5.0 in all the three lakes.Acidification consistently reduced the diversity and richness of species in taxonomic groups studied, these effects resulting from losses of species and the increased dominance of a few acidophilic taxa.Responses of experimentally-acidified lakes in north-western Ontario and atmospherically-acidified lakes in eastern Ontario were similar in most respects where records allowed comparisons to be made, notably in relation to biogeochemical processes and the disappearance of acid-sensitive biota.When the acidification of L223 was reversed, several biotic components recovered quickly. Fish resumed reproduction at pHs similar to those at which it ceased when the lake was being acidified. The condition of lake trout improved as a result of greatly increased populations of small fish, their prey. Many species of insects and crustaceans that had been extirpated by acidification returned. Assemblages of phytoplankton and chironomids have retained an acidophilic character, although their diversity during recovery is similar to that at comparable pHs during progressive acidification. As their chemistry recovered, atmospherically-acidified lakes in the Sudbury area were able to sustain recruitment by species offish, including lake trout and white sucker, with rapid increases in the diversity of invertebrate taxa. Results from both L223 and lakes near Sudbury suggest a rapid partial recovery of lacustrine communities when acidification is reversed.It is concluded that the experimental lakes responded similarly to acidification, and that experimental acidification can reliably indicate the effects of acidification attributable to acidic precipitation.