Ⅰ. General Methods and MaterialsAll reactions involving air-and moisture-sensitive reagents were carried out under an argon atmosphere. 1 H and 13 C NMR spectra were recorded on a Bruker AC-P 400 spectrometer (400 MHz for 1 H and 101 MHz for 13 C) in CDCl 3 with TMS as internal standard. Chemical shifts (δ)were measured in ppm relative to TMS δ = 0 for 1 H, or to chloroform δ = 77.0 for 13 C as internal standard. 19 F NMR spectra were recorded on the same instrument. Data are reported as follows:Chemical shift, multiplicity (s = singlet, d = doublet, t = triplet, q = quartet, m = multiplet), Coupling constants, J, are reported in hertz. Mass spectra were mearsured using Bruker microTOF-Q II (2a, 2c, 2j-2l, 2u) and Thermo Scientific DSQ II mass spectrometer (2b, 2d-2i, 2m-2p, 2v-2w). The starting materials were purchased from Acros Organics, J&K Chemicals or TCI and used without further purification. Solvents were dried and purified according to the procedure from "Purification of Laboratory Chemicals book". Thin-layer chromatography (TLC) was performed using 60 mesh silica gel plates visualized with short-wavelength UV light (254 nm). Substrates were prepared according to literature methods A 1 and literature methods B 2, 3 . Ⅱ. Typical Procedures for the Synthesis of Substrates Method ATypical procedure:Allyl bromide (16.24 mmol) was added dropwise to a solution of commercially available aniline (16.24 mmol) and K 2 CO 3 (38.97 mmol) in DMF (37 mL). The solution was heated to 80 ºC and stirred at this temperature overnight. The reaction mixture was then filtered, washed with

The catalyst showed high efficiency in the oxidation of alcohols to ketones or acids and can be recycled several times.

Background: Bell’s palsy is a widespread disease of the peripheral nervous system which causes not only physical disorders but also mental suffering as well. However, the etiological factor of Bell’s palsy is still unclear. The present study aimed to search for potential influencing factors by identifying the key genes and long non-coding RNAs (lncRNAs) involved in patients with Bell’s palsy using RNA-Seq data based on bioinformatics tools. Methods: Differentially expressed genes (DEGs) and differentially expressed lncRNAs (DELs) in patients before and after therapy, and that in normal control group were identified. The competing endogenous RNAs (ceRNAs) regulatory network was constructed by integrating lncRNA-mRNA pairs, miRNA-mRNA regulatory pairs, and miRNA-lncRNA pairs using Cytoscape. The Gene Ontology (GO) functions and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways analyses of DEGs and DELs were evaluated to explore their functions. The targeted corresponding genes and pathogens were verified by ELISA and q-PCR. Results: In the present study, hub proteins such as CXCR2 and IL1R1 in PPI network and 1039 lncRNA-mRNA co-expression pairs (eg., CYYR1-AS1-MDM2) were identified. 739 miRNA-mRNA pairs (eg., hsa-miR-147a-IL1R1), 255 miRNA-lncRNA pairs, and 363 mRNA-lncRNA co-expression pairs were included in ceRNA regulatory network. Meanwhile, CYYR1-AS1 was enriched into most pathways, including Epstein-Barr virus (EBV) infection. Subsequently, validation of neuroinflammation relevant IL1R1 and EBV showed that IL1R1 was upregulated in the serum of patients with Bell’s palsy before therapy, while EBV was not found among them. Conclusion: We hypothesized that etiological factor of Bell's palsy correlate to complex miRNA-lncRNA-mRNA interacting networks and IL1 might be involved in inflammation and immune regulation in the onset of Bell's palsy.