Medicago sativa L. (Alfalfa) was record as the Uygur Medicine herb and the common name was called Lucerne 1. Medicago sativa L. is a perennial dicotyledonous herbaceous plant belongs to leguminosae which comprises over 85 species of rarely shrubs 2,3. Medicago species are distributed in the temperate regions originated in Asia 4. The most ancient plant has been used widely such as sprouts for green salads or in the form of tablets or juices, soil improvement, animal feed, medicinal uses, etc. 5. Due to its high protein content and relatively low fiber component, the aerial parts may be used as a forage crop in the form of fodder plant. The medicinal attributes of Medicago sativa L. have been known for many years, which is used to cure entral nervous, digestive system disorders, cardiovascular, kidney pain and cough, to improve the memory, as an antiatherosclerotic, antidiabetic, antiasthmatic, antioxidant, antimicrobial, antiinflammatory and for the treatment of other ailments 4-6. Many studies showed that the most important bioactive components of the Medicago sativa L. are flavonoids, isoflavonoids, phenolic compounds, phytoestrogens, phytosterols, vitamins, volatile components 7,8. To our best of knowledge, there are limited information available on the trace elements in Medicago sativa L. seeds from Xinjiang, China.