Phenanthrene (PHE) is a common pollutant of acidic and non-acidic environments that is recalcitrant to biodegradation. Herein, Burkholderia fungorum FM-2 (GenBank accession no. KM263605) was isolated from oil-contaminated soil in Xinjiang and characterized morphologically, physiologically, and phylogenetically. Environmental parameters including PHE concentration, pH, temperature, and salinity were optimized, and heavy metal tolerance was investigated. The MIC of strain FM-2 tolerant to Pb(II) and Cd(II) was 50 and 400 mg L−1, respectively, while the MIC of Zn(II) was >1,200 mg L−1. Atypically for a B. fungorum strain, FM-2 utilized PHE (300 mg L−1) as a sole carbon source over a wide pH range (between pH 3 and 9). PHE and heavy metal metabolism were assessed using gas chromatography (GC), inductively coupled plasma optical emission spectroscopy (ICP-OES), scanning electron microscopy-energy dispersive X-ray spectroscopy (SEM-EDS), Fourier-transform infrared (FTIR) spectroscopy and ultraviolet (UV) absorption spectrometry. The effects of heavy metals on the bioremediation of PHE in soil were investigated, and the findings suggest that FM-2 has potential for combined bioremediation of soils co-contaminated with PHE and heavy metals.

Trimethoprim is one of the representative drugs within the pharmaceutical and personal care products (PPCPs) group. The photo-Fenton oxidation technology was used to degrade trimethoprim in wastewater and the extent of degradation was analyzed by using high-performance liquid chromatography, then experimentally obtained the optimal conditions. Analysis of the experimental data showed that, under the single-factor experimental conditions, the optimal conditions for degradation were a pH of 4, an H2O2 concentration of 3.0 mmol/L, an FeSO4 concentration of 0.06 mmol/L, an initial trimethoprim concentration of 0.0689 mmol/L, and an ultraviolet (UV) intensity (UVA) of 12 mW/cm2. The interaction of pH and the concentration of H2O2 and Fe2+ have been further explored, it was obtained the following response surface results through the central composite design experiment: pH = 4.56, H2O2 concentration = 0.09 mmol/L, and Fe2+ concentration = 0.09 mmol/L. Under these conditions, it can be obtained a degradation rate of 99.95% after 6 min. There were similar results for three sets of parallel experiments, indicating that these simulation conditions were feasible.

The outbreak of cyanobacterial blooms in water can cause serious harm to water supply safety and human health, which has become one of the major water environmental problems in the present world. In this paper, the representative Microcystis aeruginosa blooms were studied by the eutrophic microcosmic simulation device between natural lake system and test in laboratory scale. Toxic Microcystis aeruginosa FACHB-905 and non-toxic Microcystis aeruginosa FACHB-469 were used to simulate algal blooms in different nutrient conditions. The study of stratified EPS extracted from different vegetative and growth stages of Microcystis aeruginosa showed that high nutritional conditions can induce Microcystis aeruginosa to produce more extracellular polysaccharides, while low nutrient conditions can affect the fluorescence components in the SL-EPS (Soluble EPS) of Microcystis aeruginosa. Furthermore, the proteins in TB-EPS (Tightly bound EPS) will gradually release to SL-EPS and LB-EPS (loosely bound EPS) during the bloom, which can decrease from 34% in the initial growth period to 20%. The tryptophan-like substances in SL-EPS and TB-EPS, and the humic-acid-like substances in SL-EPS have a significant impact on the Microcystis aeruginosa blooms. Finally, all these results are expected to be of use in the study of the algal blooming outbreak early warning and control problems.

Microcystis aeruginosa is the dominant alga forming cyanobacteria blooms, the growth of which is limited by available nutrients. Thus, it is necessary to study cyanobacteria blooms and explore the growth of Microcystis aeruginosa under different nutrient conditions. In this paper, we take Microcystis aeruginosa, including toxic Freshwater Algae Culture of Hydrobiology Collection (FACHB)-905 and non-toxic FACHB-469 strains, into account. The strains were cultured using a simulation device under different nutrient conditions. Ultraviolet spectra, three-dimensional fluorescence spectra, and kinetic parameter indicators of the two species are studied. Compared to FACHB-469, the results show that the specific growth rate of FACHB-905 is much higher, in particular, FACHB-905 is the dominant species under low nutrient conditions. Furthermore, the UV spectral characteristics indicate that the molecular weight of dissolved organic matter in the culture tank of toxic FACHB-905 is greater than that of FACHB-469. Additionally, the humification index of toxic FACHB-905 is slightly higher as well, which suggests that it is more stable in the presence of dissolved organic matter during blooms. Therefore, the toxic Microcystis strain is more likely to become the dominant species in water blooms under lower eutrophic conditions and water blooms formed by the toxic Microcystis strain may be more difficult to recover from.