No abstract

Tujuan dari kegiatan pengabdian masyarakat ini adalah terwujudnya Desa Mandiri Sampah untuk menjadikan Desa Sekaran mampu mengelola dan mengolah sampah sendiri serta terciptanya nilai ekonomis bagi masyarakat. Bank Sampah yang terbentuk, menjadi salah satu solusi untuk efektifitas dan efisiensi dalam pengelolaan sampah skala rumah tangga. Bank sampah bertujuan untuk mereduksi sampah yang dihasilkan masyarakat secara terintegrasi sebagai bagian dari mata rantai siklus sampah anorganik. Metode yang digunakan dalam pelaksaanaan adalah program metode Community Developmen yaitu dengan melibatkan peran aktif masyarakat dan khalayak sasaran serta sumber daya local yang terdaat di lokasi program yang dimulai dari tahapan sosialisasi, dan pendampingan dari awal tahapan program sampai pasca program. Bank sampah berhasil didirikan dengan strategi pengolahan sampah 3R dengan tujuan agar masyarakat mampu mengubah imajinasi sebian besar orang terhadap sampah yang tidak memiliki nilai ekonomi. Selain itu terciptanya sitem aplikasi digital yang memudahkan masyarakat dalam pengangkutan dan pengolahan sampah. Keunggulan dari penggunaan sistem aplikasi ini adalah dapat mempermudah pendataan sampah yang akan disetorkan dan juga lebih praktis.

The gene MSTN is a member of the superfamily growth gene transforming growth factor β (TGF-β) that functions to suppress muscle growth. The purpose of this study was to analyze the polymorphisms of MSTN genes in the coding region (exon 1, 2, and 3) in Bali cattle kept at the Breeding Center for Bali cattle (BPTU-HPT) in Denpasar. The number of samples used was 52 heads male of Bali cattle aged 2-3 years. SNP identification was done by amplifying the coding region, exon 1, exon 2 and exon 3 MSTN genes using direct sequencing method. Analysis of MSTN gene sequences in Bali cattle was carried out by using the BioEdit and MEGA7 programs. The present study found a total of 18 SNP mutations namely point mutation that are 4 SNP (g.313 C>A, g.324 T>A, g.330 T>G, g.400 G>A) in exon 1, 1 SNP (g.2609 G>A) in exon 2, and 13 SNP (g.4821 C>A, g.4838 C>T, g.4842 A>C, g.4849 T>C, g.4868 C>A, g.4873 C>G, g.4900 T>G, 4905 C>G, g.4957 C>G, g.4959 C>A, g.4969 T>A, g.4974 T>C, g.5044 C>A) in exon 3. SNPs found in the MSTN gene in Bali cattle might be used as candidates for Marker Assisted Selection, especially in Bali cattle.

Limousin cattle (Bos Taurus) are beef cattle that have a large, long, body shape, with have advantages of fast body growth, high fertility and easy reproducibility. Myostatin gene is a family of TGF-β which plays a role in muscle growth and meat quality. This study aims to identify diversity of the Single Nucleotide Polymorphism (SNPs) Myostatine gene in the coding region in limousine cattle. Blood samples were taken from 15 limousine cattle from BPTU-HPT Padang Mangatas, West Sumatra Province. SNPs identification is finish by amplifying the coding region using the sequencing method. This study revealed 12 SNPs spread over 2 SNPs in exon 1 (c.400 G>A, c.415 C>A), 8 SNPs in exon 2 (c.2411 C>T, c.2477 G>A, c. .2489 G>A, c.2503 G>A, c.2521 G>A, c.2577 C>T, c.2609 G>A, c.2636 G>A), and in exon 3 find 2 SNPs (c .5107 C>A, c.5113 T>C) are polymorphic with allele frequencies in general being in Hardy-Weinberg equilibrium except for SNP c.2411 C>T which is not in balance. SNPs identified in the MSTN gene of limousine cattle are expected can fungtion as candidate genetic markers, especially for the perfection of beef cattle in Indonesia. Key words: Limousin Cattle, MSTN, SNP

This study aims to identify the morphometric characteristics of male Boerka goats and female Boerka goats at different age levels as a basic step of selection in breeding and improving the genetic quality of goats. A total of 25 samples of Boerka goats, namely 10 male Boerka goats and 15 female Boerka goats were used to identify qualitative and qualitative traits at the age levels of 0-6 months, >6-12 months, and >12 months. The morphometric parameters observed consisted of qualitative characteristics, namely the diversity of coat colors, the diversity of horn shapes, the diversity of the color of the legs and the diversity of the front lines. While quantitative traits consist of body weight, height, body length, and chest circumference. The results of qualitative research found that Boerka goats had a dominant diversity of brown white fur color, short and small horns, black and white leg color, and a convex head shape. The average values ​​of body weight, height, body length, and chest circumference of male Boerka goats and female Boerka goats at various age levels were not significantly different except for chest circumference with age group > 12 months