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Foodborne hydrolyzed antifreeze peptides have been widely used in the food industry and the biomedical field. However, the components of hydrolyzed peptides are complex and the molecular mechanism remains unclear. This study focused on identification and mechanism analysis of novel antifreeze peptides from Takifugu obscurus skin by traditional methods and computerassisted techniques. Results showed that three peptides (EGPRAGGAPG, GDAGPSGPAGPTG, and GEAGPAGPAG) possessed cryoprotection via reducing the freezing point and inhibiting ice crystal growth. Molecular docking confirmed that the cryoprotective property was related to peptide structure, especially α-helix, and hydrogen bond sites. Moreover, the antifreeze peptides were double-faces, which controlled ice crystals while affecting the arrangement of surrounding water molecules, thus exhibiting a strong antifreeze activity. This investigation deepens the comprehension of the mechanism of antifreeze peptides at molecular scale, and the novel efficient antifreeze peptides can be developed in antifreeze materials design and applied in food industry.

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Fujian is very wealthy in natural resources, which provides a good environment for social development and people’s life. However, with the development of economy, environmental pollution has become an importation problem in Fujian. In order to discuss the linkage relationship between economic development and environmental pollution, this paper selects the data of the secondary industry, industrial waste gas emissions, industrial waste water emissions and industrial solid waste production from 1995 to 2017 in Fujian, establishes the VAR model, explore the relationship between the development of secondary industry and environmental pollution. The results show that the association between the development of the secondary industry and environmental pollution is monotonously increasing. The industrial waste gas has become the main pollutant restricting the development of the secondary industry. Industrial solid waste has made the largest contribution to the development of the secondary industry, indicating a positive dynamic correlation.