Background: Menopause is a biological process that can cause various troublesome symptoms such as hot flashes and emotional changes but can also affect cognitive functions. Soy isoflavones (SIF) are natural compounds that gained popularity as an alternative treatment for menopausal symptoms. Aim:The aim of this work was to investigate the effect of isoflavone treatment on memory in ovariectomized female albino rats. Method: 32 female albino rats were divided into 4 groups consisting of 8 rats in each group; sham, sham+isoflavone, bilateral Ovariectomy (OVX), OVX + isoflavone. After isoflavone treatment, the animals underwent the novel object recognition test (NORT), and their performance was evaluated at the end of the experiment. Gamma amino butyric acid (GABA) and glutamate were measured in hippocampal tissues and hormonal level of follicle stimulating hormone (FSH) & 17β-estradiol (E2) were measured in serum Results: Animals with ovariectomy showed significant increase (P<0.05) in GABA and decreased glutamate in parallel with a decrease in NORT performance and decreased level of (E2). However, in the isoflavone treated group, there was a significant decrease (P<0.05) in GABA and increase in glutamate observed while there was improvement in NORT performances and an increase in E2 level.Conclusion: These results suggest that isoflavone could improve menopause associated cognitive impairment through modulating brain level of GABA and glutamate therefore; isoflavone could be an effective tool to combat this undesirable feature of the natural menopause.