With rising consumer demands, society is tapping into wastewater as an innovative source to recycle depleting resources. Novel reclamation technologies have been recently explored for this purpose, including several that optimize natural biological processes for targeted reclamation. However, this emerging field has a noticeable dearth of synthetic material technologies that are programmed to capture, release, and recycle specified targets; and of the novel materials that do exist, synthetic platforms incorporating biologically inspired mechanisms are rare. We present here a prototype of a materials platform utilizing peptide amphiphiles that has been molecularly engineered to sequester, release, and reclaim phosphate through a stimuliresponsive pH trigger, exploiting a protein-inspired binding mechanism that is incorporated directly into the self-assembled material network. This material is able to harvest and controllably release phosphate for multiple cycles of reuse, and it is selective over nitrate and nitrite. We have determined by simulations that the binding conformation of the peptide becomes constrained in the dense micelle corona at high pH such that phosphate is expelled when it otherwise would be preferentially bound. However, at neutral pH, this dense structure conversely employs multichain binding to further stabilize phosphate when it would otherwise be unbound, opening opportunities for higher-order conformational binding design to be engineered into this controllably packed corona. With this work, we are pioneering a new platform to be readily altered to capture other valuable targets, presenting a new class of capture and release materials for recycling resources on the nanoscale.

Although peptide amphiphile micelles (PAMs) have been widely studied since they were developed in the late 1990s, to the author's knowledge, there have been no reports that PAMs intrinsically fluoresce without a fluorescent tag, according to the aggregation-induced emission (AIE) effect. This unexpected fluorescence behavior adds noteworthy value to both the peptide amphiphile and AIE communities. For PAMs, intrinsic fluorescence becomes another highly useful feature to add to this well-studied material platform that features precise synthetic control, tunable self-assembly, and straightforward functionalization, with clear potential applications in bioinspired materials for bioimaging and fluorescent sensing. For AIE, it is extremely rare and highly desirable for one platform to exhibit precise tunability on multiple length scales in aqeuous solutions, positioning PAMs as uniquely well-suited for systematic AIE mechanistic study and sequencespecific functionalization for bioinspired AIE applications. In this work, the author proposes that AIE occurs across intermolecular emissive pathways created by the closely packed peptide amide bonds in the micelle corona upon self-assembly, with maximum excitation and emission wavelengths of 355 and 430 nm, respectively. Of the three PAMs evaluated here, the PAM with tightly packed random coil peptide conformation and maximum peptide length had the largest quantum yield, indicating that tuning molecular design can further optimize the intrinsic emissive properties of PAMs. To probe the sensing capabilities of AIE PAMs, a PAM was designed to incorporate a protein-derived phosphate-binding sequence. It detected phosphate down to 1 ppm through AIE-enhanced second-order aggregation, demonstrating that AIE in PAMs leverages tunable biomimicry to perform protein-inspired sensing.

recommended 2 hours per day of physical activity is obtained by less than 10% of preschoolers nationwide. 10,11 ECEs present an opportunity to add physical activity and reduce sedentary behavior in children, because more than 80% of children spend some time in childcare settings by the age of 3 years.12 However, physical activity is highly variable within and across these settings.

With rising consumer demands, society is tapping into wastewater as an innovative source to recycle depleting resources. Novel reclamation technologies have been recently explored for this purpose, including several that optimize natural biological processes for targeted reclamation. However, this emerging field has a noticeable dearth of synthetic material technologies that are programmed to capture, release and recycle specified targets, and of the novel materials that do exist, synthetic platforms incorporating biologically inspired mechanisms are rare. We present here a prototype of a materials platform utilizing peptide amphiphiles that has been molecularly engineered to sequester, release, and reclaim phosphate utilizing a stimuli-responsive pH trigger, exploiting a protein-inspired binding mechanism that is incorporated directly into the self-assembled material network. This material is able to sequester completely and controllably release phosphate for multiple cycles of reuse. We have determined by simulations that the binding conformation of the peptide becomes constrained in the dense micelle corona at high pH such that phosphate is expelled when it otherwise would be preferentially bound. However, at neutral pH, this dense structure conversely employs multi-chain binding to further stabilize phosphate when it would otherwise be unbound, opening opportunities for higher-order conformational binding design to be engineered into this controllably packed corona. With this work, we are pioneering a new platform to be readily altered to capture other valuable targets, presenting a new class of capture and release materials for recycling resources on the nanoscale.

No abstract