Batok glutinous rice especially local varieties, is one of the agricultural commodities that can be used as a source of food. Besides having a good taste, batok glutinous rice is also not easily attacked by pests and rice seeds easily obtained. However, obstacles in the utilization of glutinous rice for local varieties are long harvest time and low production. Therefore, seed priming is carried out by utilizing biofertilizer derived from the rhizosphere fungus, Trichoderma spp. Trichoderma is a function that is classified as Plant Growth Promoting Function (PGPF) which is able to stimulate plant growth by producing growth regulators. This study aims to determine the response of the germination of batok glutinous rice seeds against of Trichoderma spp. This research is an experimental study using a completely randomized design with 7 treatments and 3 replications. The treatment given was soaking seeds with 6 types of Trichoderma spp. isolates and 1 control soaked with aquadest. The data obtained were analyzed statistically using ANOVA. If there are significant differences, further tests of DMRT are carried out with α = 5%. The results obtained indicate that Trichoderma spp. no significant effect on the percentage of germination and vigor index, but significant effect on the germination speed with RE isolates is the best isolate in increasing the seed germination speed.