Abstract:Bilirubin is important in disease diagnosis and it is considered as a photosensitive molecule. Therefore, precautions are important while analyzing samples to be measured bilirubin in the laboratory to avoid under-estimations. Hence, this study was designed to investigate the effect of temperature, sun-light and artificial light on the stability of serum bilirubin. Whole blood was collected from 34 apparently healthy subjects and serum was separated. To determine the effect of temperature serum was exposed to -4°C and 33°C for 2h and 24h. To investigate the effect of light the serum specimens were exposed to sun-light for 6h and two CFL bulbs, 15W each for 24h. Bilirubin concentrations were measured by dimethylsulfoxide method using automated Mindray BF 300 analyzer. The differences between base-line bilirubin value and other subsequent measurements were analyzed by post-hoc Dunnets test. There were significant reduction in all the bilirubin fractions in the samples exposed to sun-light for 6h and only total and indirect bilirubin fractions significantly had been reduced in the samples exposed to artificial light. The same effect was observed in the normobilirubinaemic samples exposed to 33°C for 24h but in hyperbilirubinaemic samples all the bilirubin fractions had been reduce significantly. There was no significant reduction of bilirubin in samples stored at -4°C for 2h and 24h and at 33°C for 2h. Therefore, serum samples to be measured bilirubin can be stored at -4°C up to 24h and 33°C up to 2h with light protection, without significant changes in bilirubin concentrations.