We investigate the roles of mTor signaling in the formation of Müller glia-derived progenitor cells (MGPCs) in the chick retina. During embryonic development, pS6 (a readout of active mTor signaling) is present in early-stage retinal progenitors, differentiating amacrine and ganglion cells, and late-stage progenitors or maturing Müller glia. By contrast, pS6 is present at low levels in a few scattered cell types in mature, healthy retina. Following retinal damage, in which MGPCs are known to form, mTor signaling is rapidly activated in Müller glia. Inhibition of mTor in damaged retinas prevented the accumulation of pS6 in Müller glia and reduced numbers of proliferating MGPCs. Inhibition of mTor had no effect on MAPK signaling or on upregulation of the stem cell factor Klf4, whereas Pax6 upregulation was significantly reduced. Inhibition of mTor potently blocked the MGPC-promoting effects of Hedgehog, Wnt and glucocorticoid signaling in damaged retinas. In the absence of retinal damage, insulin, IGF1 and FGF2 induced pS6 in Müller glia, and this was blocked by mTor inhibitor. In FGF2-treated retinas, in which MGPCs are known to form, inhibition of mTor blocked the accumulation of pS6, the upregulation of Pax6 and the formation of proliferating MGPCs. We conclude that mTor signaling is required, but not sufficient, to stimulate Müller glia to give rise to proliferating progenitors, and the network of signaling pathways that drive the formation of MGPCs requires activation of mTor.

Small- and medium-sized entities (SMEs) continuously face the challenge of proper implementation of accounting packages. Not managing the package installation and configuration processes properly will result in the accounting software not working properly and not achieving the strategic goals of the SME. The purpose of this study was to examine, by means of an extensive literature review, the potential of PRINCE2 (Projects in Controlled Environments) as a project-management framework for application by SMEs during the implementation of new accounting software. The potential of scaling down PRINCE2 to a version usable to SMEs was investigated. PRINCE2 was found to be a suitable framework for use by SMEs in the implementation of accounting software packages, but only if tailored properly to incorporate the specific needs of the SME and adjusted to specifically address strategic alignment issues. Recommendations by other researchers on the weaknesses and shortcomings of the PRINCE2 framework were also investigated and included in the development of a new model for SMEs. This article contributes to the academic literature by providing it with a standard tailored version of PRINCE2 that can be used by SMEs in the implementation of new accounting software.

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Both high cis‐1,4‐polybutadiene and high trans‐1,4‐polybutadiene were found to isomerize when heated in the presence of elemental sulfur. The kinetics of the sulfur induced isomerization of high cis‐1,4‐polybutadiene were studied over a wide range of temperature and sulfur concentration and found to obey a first‐order kinetic expression having an effective rate constant directly proportional to the initial sulfur concentration. The apparent activation energy was found to be 30.1 ± 0.4 kcal./mole. Isomerization was also found to be induced by dicumyl peroxide. A comparison of the mechanisms involved in the sulfur and peroxide induced isomerization indicate a basic difference in the nature of the initial interaction of the double bond with the sensitizing species. The initial interaction of the sulfur and the double bond is pictured as a π‐complex that decomposes to a freely rotating radical, whereas the peroxide induced isomerization is presumed to involve direct radical addition to the double bond.

Introduction: We discuss the phenotypic features of a clinically significant blood culture isolate that was ultimately identified as Pasteurella bettyae, with important laboratory learning points. Laboratory diagnosis: The blood culture from a female patient with septic shock from an unknown source grew a Gram-negative, oxidase-positive, coccobacillus that was initially identified by API® NH as Haemophilus influenzae (%ID=99.8; T=0.75). A respiratory source was therefore investigated for. The MALDI-TOF result, which was returned to the laboratory 4 weeks after blood culture became positive, revealed the isolate to be Pasteurella bettyae (score=2.305). P. bettyae is an unusual organism associated with gynaecological infections, such as Bartholin’s abscess. Local source control, such as incision and drainage of a Bartholin’s abscess, may accelerate resolution of sepsis. The laboratory misclassification of P. bettyae as H. influenzae using API® NH may lead to misidentification of the source of infection. Learning points: 1. Correctly identifying P. bettyae as the cause of a Gram-negative coccobacillus bacteremia can prompt suspicion of a gynaecological source of sepsis. 2. P. bettyae and H. influenzae, as members of the same family Pasteurellaceae, have overlapping morphological and biochemical features that can lead to laboratory misclassification. A catalase test and x+v plates can clearly differentiate the isolates (discussed further in poster). It is recommended that laboratories perform both tests, with MALDI-TOF confirmation if possible, rather than rely solely on the result of an API® NH.