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CLARK, WILLIAM A. (American Type Culture Collection, Washington, D. C.). Comparison of several methods for preserving bacteriophages. Appl. Microbiol. 10:466-471. 1962-A wide variety of bacteriophages were processed and stored under different conditions to compare methods for long-term preservation. Specimens were stored for 2 years at room temperature (24 to 28 C) and at 4 C as broth lysates in 50 % glycerol, dried, and freezedried. Titers determined after processing indicated that, of the broth, glycerol, and freeze-dry methods, freezedrying was most damaging to the phages tested, glycerol less damaging, and the broth method least damaging. After 2 years, titers of broth lysates were generally higher than those of glycerol or freeze-dried preparations. Dried preparations generally did not prove satisfactory. Preparations stored at 4 C showed better titers than those kept at room temperature. All titers declined with time regardless of the conditions of preservation.

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