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PLANT PHYSIOLOGY dant could be measured simultaneously as described earlier (6). These electrode vessels were very useful for making potentiometric measurements under different gas phases since the large surface area of the reaction mixture (3.3 cm2 per ml with a 3.0 ml system) permitted rapid attainment of equilibrium conditions. The gassing, shaking, and temperature control procedures were carried out with the standard Warburg apparatus (6, 7). Electrodes were inserted through sleeve-type serum bottle stoppers. The sidearm was provided so that parts of the reaction system could be kept initially separated if necessary, while the vent permitted passin, any desired gas mixture through the vessel. SUMMARYA simple, battery-operated, impedance matching circuit for connecting a platinum-calomel electrode system to a Brown recorder for use in recording oxidation-reduction potentials from biological systems is described. Application to the study of the Hill reaction (photolysis of water by isolated chloroplasts) is discussed. Reaction cells and modified Warburg vessels for making potentiometric measurements on photosynthetic systems are discussed.

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Nicotinonitrile product: nicotinonitrile

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