The presentation of DVT and that of a Baker's cyst are similar enough to be difficult to distinguish by clinical examination. Careful examination of the popliteal fossa should be performed during venous duplex examinations regardless of the indication for the study.
A gas-liquid chromatographic (GLC) method is described for the determination of diethylstilbestrol (DES) and its glucuronide (DES-G) in cattle tissues. Muscle, liver, and kidney samples are extracted with methanol and centrifuged. An aliquot of extract equivalent to 50 g tissue is evaporated to remove methanol and hydrolyzed overnight, using β-glucuronidase. In the resulting sample, free DES is purified by alkaline liquid-liquid extraction and is reacted in base with dichloroacetyl chloride to form the DES diester. The diester is measured by GLC, using a 5% UC W-98 column and electron affinity detection. Fat samples are assayed for free DES by extracting with chloroform and processing as other tissues, beginning with the alkaline purification. Residues from tissues of steers fed DES, assayed by both GLC and bioassay procedures, showed excellent agreement. In these studies the GLC procedure had a sensitivity of about 0.001 ppm. Lean and fat contained no DES residue when animals were slaughtered at 0 time off medicated feed. Liver and kidney contained residues on the order of 0.02 ppm at 0 time. The DES declined to nondetectable levels in liver and kidney by 96 hr after withdrawal. The assay procedure has been used successfully on a routine basis for several months.
Addressing the health needs among refugee populations requires an approach that recognizes social, cultural and language issues and has a platform for interventions that actively use the strengths and competencies from a variety of healthcare disciplines. An interprofessional practice model was implemented in 2012 at the University of Louisville as part of a program aimed at providing age-appropriate vaccines for newly arriving refugees in the Louisville community. During 2012-2014, the program involved more than 35 faculty, 22 residents and fellows, 278 students, 28 support staff and 16 researchers in collaborative work representing nursing, medicine, public health, pharmacy, and social services disciplines in addition to students and faculty from business and engineering. As of early 2015, more than 20,000 doses of vaccine have been safely provided to more than 5,000 refugees. The processes and outcomes have been deemed as beneficial by the refugees and healthcare providers. This type of interprofessional practice model may serve as a deliberative learning platform for other refugee health services.
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