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About the same time Amory * (1868) published the results of his Studies on the toxicity of caffein in cats, dogs, rabbits, and pigeons. In all cases very large doses were introduced directly into the stomach by means of a temporary gastric fistula. Ten grains given in meat to a dog caused restlessness, but no other symptoms. Doses of 30 grains and above were invariably fatal. Seventy-three grains given to a cat caused death within 20 minutes. From observations on frogs, guinea pigs, rabbits, and on one dog, Leven^^(1868) concluded that caffein which he gave in the form of the citrate in doses of 10 mg to frogs, from 150 to 200 mg to guinea pigs, and three to four times the latter amount to rabbits, stimulates the central nervous system and the voluntary, cardiac, and smooth muscles. He found that 0.9 gram caffein was fatal for a rabbit when injected subcutaneously, while 1 gram of the citrate was not toxic for a dog of medium size. Caffein applied directly to muscle fiber causes tetanus and destroys muscular contractility, while a nerve fiber similarly treated loses its irritability. According to Johansen ** (1869), caffein acts directly on the muscular fiber. After the subcutaneous injection of 0.02 gram of caft'ein into frogs, he observed contraction of the muscles at the site of injection, then contraction of the anterior extremities, and finally the posterior extremities become rigid and extended. Johansen obsei-ved muscular rigidity after caffein, even after curara was injected, or after ligating the vessels, or cutting the nerves which supply the muscles. He also observed that large doses of caffein diminish muscular irritability. When cardiac muscle was poisoned with caffein, microscopical examination showed that the striations disappeared. Johansen also states that reflexes disappear after caffein poisoning. He never observed tetanus in frogs, but reported tonic and clonic convulsions a.s (Doses of 147 to 107 inK of cafloln per kilo were employod in these experiments.] Rabbit 3S2. Bclgicin hare, female. Weight, 1,070 grains. Diet, oats. March 25: 8.5 cc 2 per cent caffein (158 mg per kilo) injected subculaneously at 2.15 p. m.; 4 p. in., reflexes increased; 5.45 p. m., increases of reflexes still more marked. March 2(;: Rabbit looked normal; no symptom.s observed. Rabbit.1^1. Belgian hare, female. Weight, 1,170 grams. Diet, oaLs. March 25: 2.15 p. m., 9 cc 2 per rout caffein (153 mg per kilo) injected subcutaneously; 4 p. m., reflexes increa.sed; 5.45 p. ni., condition the same. March 2(j: Rabbit looks normal; no symptoms observed. Rabbit 3JS. Belgian hare, female. Weight, 1,300 grams. Diet, oats. March 25: 9 cc 2 percent caffein injected subcutaneously (1-50 mg per kilo); 4 p. m., reflexes increased; 5.45 p. m., reflexes increased but not markedly. March 26: No symptoms; rabbit looks normal. Rabbit 322. White female. Weight, 1,065 grams. Diet, oats. March 17: 8 cc 2 per cent caffein (150 mg per kilo) injected subculaneoiisly at 11.55 a. m.; 12. .55 p. m., reflexes increased, but no tetanus nor any oilier...

The experiments were carried out on frogs, rabbits, cats and dogs. The alcohols were administered in various concentrations and were given by mouth, injected subcutaneously, or into the peritoneal cavity. The toxicity of amyl alcohol was in all cases much greater than that of ethyl alcohol. The difference in the toxicity of ethyl and amyl alcohol was even more marked in subacute intoxication. The experiments on frogs showed that the minimum fatal toxic dose of amyl alcohol is from one eighth to one seventh that of ethyl alcohol, while the toxic dose of amyl alcohol for the rabbit is only about one fourth to one half that of ethyl alcohol.other experiments in which from 25 to 50 C.C. of 2 per cent. ethyl alcohol caused little noticeable change or only a slight fall of blood pressure, after the injection of the same quantities of amyl alcohol, the maximum fall of blood pressure amounted to 40 and 95 millimeters of mercury. The recovery was also much slower in all cases after amyl alcohol and was much more gradual than the fall of blood pressure. Experiments with from 3 to I I C.C. of 2 per cent.

there was no mark at the point of inoculation. Cultures from the brain and blood yielded a pure growth of the greenproducing streptococcus. The

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