Summary. Hypermethylation of p15 and p16 genes was determined in 32 patients with agnogenic myeloid metaplasia(AMM), also known as idiopathic myelofibrosis (MF). These included 10 patients in leukaemic transformation phase. Using polymerase chain reaction‐based methylation analysis assay methods, with substantiation using Southern blot analysis, the study showed no hypermethylation of p15 or p16 genes in the chronic phase of AMM, but p15 gene hypermethylation was found in four patients (40%) and p16 gene hypermethylation in two patients (20%) when they were in leukaemic transformation stage. Furthermore, two of the patients in leukaemic transformation were found to have both p15 and p16 gene hypermethylation, demonstrating possible multiple gene hypermethylation in the same patient. Thus, hypomethylation agents for treating patients with AMM in leukaemic transformation may be appropriate for future trials.

The Industrial Technology Research Institute has developed a 30 kWth moving bed chemical looping system for hydrogen production. Methane is supplied as a fuel to the reducer, and steam is supplied to the oxidizer to produce hydrogen. An iron-based oxygen carrier is used circulating between the reactors. In this study, the mechanical strength, attrition rate, and conversion rate of the oxygen carrier were tested at two Fe2O3 levels of content (40 and 60 wt %) calcined at temperatures of 1100 and 1300 °C. The oxygen carrier with the lower iron content presented the higher conversion rate of 72% at both calcination temperatures. The mechanical strength (170.5 N) and attrition rate (0.3%) of the oxygen carrier with a low Fe2O3 content were higher at the higher calcination temperature. Further, the oxygen carriers were circulated in the 30 kWth moving bed chemical looping system under ambient and high-temperature conditions. The oxygen carrier with two Fe2O3 content levels (40 and 60 wt %) at the calcination temperature of 1200 °C was employed in the attrition rate test conducted in the chemical looping system. Under the ambient temperature conditions, the average attrition rate of the oxygen carrier with a lower Fe2O3 content was lower (0.16%) than that of the oxygen carrier with a higher Fe2O3 content (0.24%). Moreover, the test results show the operating temperature has a lower level of influence on the attrition rate of the oxygen carrier.