Background and objectiveBiomarkers are important tools for prompt diagnosis of cancer. This study aimed to identify reliable biomarkers for clinical applications in the diagnosis of gastric cancer and lymph-node (LN) metastasis.MethodsBetween 1 December 2014 and 31 December 2015, we prospectively collected samples of gastric-cancer tissues, corresponding matched-pair normal gastric mucosa, and their peri-gastric metastatic and non-metastatic LNs to identify quantitatively reliable genes using quantitative real-time polymerase chain reaction. Relative quantity (RQ) was used to calculate the mRNA expression levels of our target genes. Statistics were calculated using one-way analysis of variance (ANOVA) and Tukey’s multiple comparison test. Analytical graphs were plotted using GraphPad Prism.ResultsOf nine assessed genes, the mRNA levels of inhibin beta A (INHBA) and secreted phosphoprotein 1 (SPP1) were most consistently highly expressed in tumor tissues by 15.4- and 15.6-fold, respectively, as compared with normal tissues (P < 0.001), with 91.3% sensitivity and 95.7% specificity (receiver operating characteristic [ROC] curve area = 0.974) for the former and 82.6% sensitivity and 87.0% specificity (ROC curve area = 0.924) for the latter. Further analysis revealed no differentiating significance of SPP1 mRNA expression between metastatic and non-metastatic LNs (P = 0.470). In contrast, the INHBA mRNA level was up-regulated 4.1-fold in metastatic LNs (P < 0.001), with 80.0% sensitivity and 81.5% specificity (ROC curve area = 0.857), and was also able to successfully differentiate between more severe disease conditions, T3 and T4 (P = 0.003), M0 and M1 (P = 0.043) and different histological variants (intestinal type vs diffuse type, P = 0.019).ConclusionsOur results showed that INHBA was the most optimally reliable biomarker for diagnosing gastric cancer and LN metastasis.

Globally, in-home drug-storage compliance is often unsatisfactory, especially among older adults, and can lead to negative health outcomes. This study used a cross-sectional and descriptive design to examine in-home drug-storage compliance among older adults. Convenience sampling was used to recruit 117 older adults in Hong Kong. An in-home drug-storage checklist was used to assess the in-home drug-storage environments (light, temperature, and humidity) and drug-storage methods (drug safety, packaging, information, and expiration) of each older adult. The findings showed that Hong Kong older adults’ overall compliance rate in drug storage was 87.25%, and their compliance rate for drug-storage methods (84.59%) was lower than that for drug-storage environments (97.02%). Older adults who were of advanced age, who were less educated or who lived alone demonstrated lower in-home drug-storage compliance. This indicates the need to revise existing health-education strategies to encourage in-home drug-storage compliance. Healthcare professionals should assess older adults’ drug-storage compliance to identify less compliant subgroups and deliver specific drug-storage support as required. Family members should also be involved in this process.