The ethyl acetate extract of F. foveolata showed the strongest antibacterial activity with minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values of 19.5-39.0 and 39.0-156.2 µg/mL, respectively. On the basis of an antibacterial assay-guided isolation, seven antibacterial compounds, including 2,6-dimethoxy-1,4-benzoquinone (1), syringaldehyde (2), sinapaldehyde (3), coniferaldehyde (4), 3β-hydroxystigmast-5-en-7-one (5), umbelliferone (6), and scopoletin (7), were purified. Among these isolated compounds, 2,6-dimethoxy-1,4-benzoquinone (1) exhibited the strongest antibacterial activities against S. pyogenes, S. mitis, and S. mutans with MIC values of 7.8, 7.8, and 15.6 µg/mL, and MBC values of 7.8, 7.8, and 31.2 µg/mL, respectively. In addition, this is the first report of these antibacterial compounds in the stems of F. foveolata.

An antibacterial assay-guided isolation of the crude ethyl acetate extract from Ficus foveolata stems afforded four compounds, including a tetrahydronaphthalene lignanamide, foveolatamide (1), together with two known lignanamides, flavifloramide B (2) and N-trans-grossamide (3), and a known phenolic amide, N-trans-feruloyltyramine (4). The structures of these compounds were established on the basis of NMR spectroscopic and MS techniques. Among the isolated compounds, only 1 showed satisfactory antibacterial activities against Streptococcus pyogenes, with an MIC and MBC value of 45 µM. This is the first report of these four compounds from the stems of F. foveolata.

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