Methotrexate commonly used to treat cancer and causes reproductive damage in mice. The aim of this research is to study the effects of salvia aqueous extract on some parameters of sperms and histopathological changes in testis of mice treated with methotrexate. Twenty-four adult albino male mice were divided into three groups, (8 mice in each group) First group was negative control received normal saline intraperitoneally, while second group was treated with50 μg/kg, for 35 days intrapritoneally (ip). The third group was treated with methotrexate (50 μg/kg) also (ip) and extract of salvia 85 mg/kg for 35 days. The results showed significant (P<0.05) decrease in sperms motility , increased percentage of dead sperms and abnormalities of sperms in mice treated with MTX, there was significant (P<0.05) decrease in diameters of seminiferous tubules, primary spermatocytes and spermatids, increased interstitial spaces as compared with control, while in the third group result showed significant (P<0.05) increase in sperms motility , decreased percentage of dead and abnormalities of sperms and significant (P<0.05) increase in diameters of seminiferous tubules, primary spermatocytes and spermatids and decrease interstitial spaces compared with first and second groups.