Only minimal differences between endogenous and glucose respiration were demonstrable with 0.03 M phosphate buffer at pH 7. The addition of 1 ml of trace metals (Table 1) or a modified Ringer's solution (Table 2) enhanced glucose respiration markedly. The a priori hypothesis that magnesium ions were largely responsible for this stimulation was tested by adding 1 ml of the trace-metal mixture and 38.2 mg MgSO4 .7H20 to 100 ml 0.03 M phosphate buffer at pH 7. This variation proved to be superior to the previous modifications, and RQ's within 5% of theoretical were obtained with several carbon sources. With this buffer, results represented in Fig. 1 and 2 were achieved. They indicate that several carbohydrates and inter-mediates of carbohydrate metabolism were oxidized by C. longisporum without immediate prior experience. Inspection of these curves also suggests the ability of the fungus to adapt to certain of these other substrates. It is noteworthy that earlier in this investigation phosphate concentration appeared not to influence the glucose respiration of C. Iongisporum. Exhaustive shaking of the mycelium in distilled water was required before the need for phosphate became manifest, suggesting intracellular metaphosphate stores. This study would suggest that other filamentous fungi may be studied similarly after their specific metal requirements for manometric work have been established.

A method for testing nonsterile pharmaceutical preparations for their microbial content is described. As far as possible, only solid culture media were used to obtain quantitative results. Aqueous and water-soluble products were tested with membrane-filter techniques. Nonfilterable products were first emulsified or suspended and the homogenate was used for examination. In both procedures, the total number of colonies is determined for aerobic bacteria and fungi. Tests for certain undesirable microbial groups were conducted with selected media. The method described is applicable for finished products, bulk products, raw materials, and active ingredients.

Zusammenfassung Innerhalb 6 Monaten des Jahres 1966 erhielten die Autoren in zwei Fällen Bronchial‐und Mediastinallymphknoten von Schlachtkühen mit Tuberkulose‐ähnlichen Veränderungen zur Untersuchung. In beiden Fällen konnte Tuberkulose ausgeschlossen werden. Hingegen ließen sich in den Läsionen Hyphen nachweisen, die für Schimmelpilze der Familie Mucoraceae typisch waren. In einem Fall gelang es, aus dem Infektionsherd Mucor pusillus zu isolieren. Die Eigenschaften dieses Erregers und die wesentlichen Kriterien für die Differenzierung werden beschrieben. Résumé En 1966, en l'espace de six mois, les auteurs observèrent chez deux vaches abattues des lésions d'aspect tuberculeux au niveau des ganglions bronchiques et médiastinaux. Dans les deux cas les examens de laboratoire permirent d'exclure une tuberculose. Par contre, il fut décelé dans les lésions des hyphes de moisissures présentant caractères des Mucoraceae. Dans le second cas on réussit à isoler Mucor pusillus dans les foyers d'infection. Les auteurs décrivent les caractères et les principales méthodes d'identification de cet agent pathogen.

A method for testing nonsterile pharmaceutical preparations for their microbial content is described. As far as possible, only solid culture media were used to obtain quantitative results. Aqueous and water-soluble products were tested with membrane-filter techniques. Nonfilterable products were first emulsified or suspended and the homogenate was used for examination. In both procedures, the total number of colonies is determined for aerobic bacteria and fungi. Tests for certain undesirable microbial groups were conducted with selected media. The method described is applicable for finished products, bulk products, raw materials, and active ingredients.

No abstract