Introduction Nucleus pulposus (NP) replacement offers a minimally invasive disc regeneration treatment alternative to traditional fusion or total disc replacement. An ideal NP replacement material should maintain motion and disc height, thus slowing down further disc degeneration.1 Various biomaterials have been investigated for their use as NP replacement, including hydrogel and nonhydrogel scaffolds, while no direct comparison study has been performed. Recently, a novel fibrin-hyaluronan (FBG-HA) conjugate hydrogel was developed to mimic native NP extracellular matrix. In addition, a novel polyurethane (PU) scaffold with swelling capability in situ was designed to restore native disc thickness and mechanical properties. The present study aimed to assess the FBG-HA hydrogel, the PU scaffold, and the combination of both in an organ culture system with dynamic load for the purpose of NP replacement. Materials and Methods FBG-HA conjugate solution was synthesized with 235 KDa HA at FBG/HA w/w ratio of 17:1 via a two-step procedure as described elsewhere.2 FBG-HA hydrogels were prepared by mixing two-thirds volume of FBG-HA conjugate solution with one-third volume of thrombin solution (5.2 U/mL) and allowed to polymerize at 37°C for 20 minutes. The PU scaffold was designed to have a flat discoid/ravioli shape that is composed of a based core with swelling capacity. Electrospinning nanofibers technology was used to manufacture an envelope sheet using a blend of polyurethane carbonates (ChronoFlex and HydroMed) that were dissolved in a mixture of dimethylformamide and dioxane. The envelope was cut to discs at diameter of 6 mm. The PU hydroMed was dissolved in 95% ethanol and dried to form a film. The film was cut to discs at diameter of 3 mm. One film disc was placed between two envelope discs and the circumference was heat welded to form a scaffold with ravioli shape. Caudal bovine intervertebral discs (IVDs) with endplates were obtained from calves (4-8 months) directly after slaughter. The discs were nucleotomized by incisions through the endplate. A 4-mm endplate core and the NP tissue below the endplate stopper were removed. The nucleotomized region was refilled with either (1) 50 to 80 µL FBG-HA hydrogel, (2) dry PU scaffold, or (3) PU scaffold surrounded by FBG-HA hydrogel. To close the defect, the removed endplate stopper was re-inserted, and the crack between the stopper and the remaining endplate was sealed by polymethyl methacrylate. Empty discs served as negative controls. To assess the mechanical compatibility of the different biomaterial implants, dynamic compressive stiffness modulus and disc height were measured for each disc at different time points: Intact, after nucleotomy, after refilling with biomaterial and free swelling culture overnight, after 3 hours dynamic load at 0 to 0.1 MPa, 0.1 Hz within a bioreactor system,3 and after free swelling recovery overnight. Results Dry PU scaffold was able to trap water and swell in situ already after 24 hours culture in the disc, as indicated by weight ...
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
customersupport@researchsolutions.com
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
Copyright © 2025 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.