Photosynthetic heat tolerance (P HT ) is a key predictor of plant response to climate change. Mangroves are an ecologically and economically important coastal plant community comprised of trees growing at their physiological limits. Mangroves are currently impacted by global warming, yet the P HT of mangrove trees is poorly understood.• In this study, we provide the first assessment of P HT in 13 Asian mangrove species, based on the critical temperature that causes the initial damage (T Crit ) and the temperature that causes 50% damage (T 50 ) to photosystem II. We tested the hypotheses that the P HT in mangroves is: (i) correlated with climatic niche and leaf traits, and (ii) higher than in plants from other tropical ecosystems.• Our results demonstrated correlations between P HT and multiple key climate variables, the palisade to spongy mesophyll ratio and the leaf area. The two most heat-sensitive species were Kandelia obovata and Avicennia marina. Our study also revealed that mangrove trees show high heat tolerance compared to plants from other tropical ecosystems.• The high P HT of mangroves thus demonstrated a conservative evolutionary strategy in heat tolerance, and highlights the need for integrative and comparative studies on thermoregulatory traits and climatic niche in order to understand the physiological response of mangrove trees to climate change-driven heatwaves and rising global temperatures.

Cluster of differentiation antigen 14 (CD14) plays a crucial role in the inflammatory response to lipopolysaccharide (LPS), which interacts with TLR4 and MD-2 to enable cell activation, leading to inflammation. Several studies have proved that upstream inhibition of bacterial LPS/toll-like receptor 4 (TLR4)/CD14-mediated inflammation pathway is an effective therapeutic approach for attenuating damaging immune activation. In this study, to explore the effect of CD14 down-regulation on TLR4 signal conductive-related genes expression after stimulation by LPS, five CD14 shRNA (319/421/755/970/1041) sequences and a negative control sequence (NC-1864) were synthesised and used to construct lentiviral recombinant plasmid pSicoR-GFP-shRNA. Lentiviral recombinant plasmids of pSicoR-GFP-shRNA and fusion expression vector of pDsRed-N1-buffalo CD14 were co-transfected into HEK293 using liposome. At 72 h after transfection, the expression of exogenous buffalo CD14 mRNA was reduced at different level for all shRNA plasmids, in which shRNA-1041 had the highest interfering efficiency by RT-qPCR and fluorescence-activated cell sorting analysis. Then, buffalo peripheral blood monocyte/macrophage was purified and infected by the CD14 shRNA lentivirus. After 7 days of infection, the cells were stimulated by 1 µg mL–1 LPS for 3 h, then the mRNA expression level of CD14, TLR4, IL-6, and TNF-α transcripts in the cells were detected by the RT-qPCR method. After stimulation by LPS, the expression of endogenous CD14 was significantly reduced by CD14 shRNA-1041, the mRNA expression level of TLR4, IL-6, and TNF-α genes was also significantly down-regulated in comparison with control group (P ≤ 0.01). In conclusion, the selected CD14 shRNA-1041 cannot only inhibit the expression of endogenous CD14 mRNA in buffalo peripheral blood monocyte/macrophage, but also downregulate the mRNA expression of CD14, TLR4, IL-6, and TNF-α. The above results demonstrate that knockdown of endogenous CD14 has obvious coordination effects on the signal conductive function of TLR4 after stimulating by LPS, and shRNA technology will provide a new way to prevent endotoxin-related diseases in livestock. This work was supported by the National Transgenic Project (2009ZX08007-009B), Guangxi natural science funding (2012GXNSFCB053002), and funding of State Key Laboratory of Subtropical Bioresource Conservation and Utilisation (KSL-CUSAb-2012-02).

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