During the summer of 2006, pepper plants ( Capsicum annuum ) cvs Atol, Olimpus, Bjanca and Verbrana, showing virus-like symptoms, were observed in a commercial field in SE Slovenia. Discolorations, rings or dark bumps on fruits resulted in almost a 100% loss of the yield in some areas. Symptoms on leaves were expressed as arabesques or concentric rings. In autumn, infected plants expressed less severe symptoms and produced symptomless fruits.Infected leaves and fruits, with various symptoms, were tested by ELISA for the presence of BBWV 1 ( Broad bean wilt virus 1), BBWV 2 ( Broad bean wilt virus 2), CSNV ( Chrysanthemum stem necrosis virus ), CMV ( Cucumber mosaic virus ), INSV ( Impatiens necrotic spot virus ) and TSWV ( Tomato spotted wilt virus ). Positive results were obtained only for BBWV 1 in all samples collected from the epidemic area. Immuno-serological electron microscopy (ISEM) (antibodies from DSMZ, Germany) of crude sap extracts confirmed BBWV 1 infection. RT-PCR using BBWV 1 specific primers B1/B2 (Rubio et al ., 2002) produced an amplicon of approximately the expected size (500 bp) from extracts from infected but not healthy plants. The sequence of the amplicon was determined (GenBank Accession No. EF405826). BLAST analysis of a 334 bp segment, encompassing part of the two coat proteins genes, showed approximately 80% nt identity with various BBWV 1 isolates. Amino acid identities ranged from 90 to 92% with BBWV 1 isolates and from 54 to 58% with BBWV 2 isolates. Sap of plants with symptoms was used for mechanical inoculation of test plants. Local and systemic symptoms were observed on pepper, Chenopodium quinoa , Nicotiana rustica , and N. tabacum cv. White Burley. No symptoms were observed on N. clevelandii , N.benthamiana and Lycopersicon esculentum . BBWV 1 was also identified in one out of six weed species belonging to the family Cichoriaceae, expressing mild mosaic symptoms and growing near the epidemic area. This is the first report of BBWV 1 in Slovenia.
There is a loading process after the projectile of the cased telescoped ammunition (CTA) fired in the chamber, which has important influence on the stability of interior ballistics, the firing accuracy and the gun barrel life. In order to study the characteristics of velocity and attitude in the initial motion of the projectile of the CTA, a special projectile for the initial ballistic test was designed, and an initial ballistic test system based on the high-speed camera was built. The loading process after firing was observed and the loading velocity was measured. The results show that the loading speed of the projectile is about 30m/s. The initial ballistic parameters of the projectile can be studied quantitatively and the motion attitude of the projectile can be examined qualitatively by using the initial ballistic test system based on the high-speed photography.
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