Mercury (Hg) is one of the major pollutants in soils because of the annual import of toxic Hg into the agricultural lands. The aims of the present studies are to investigate the effect of Hg on chlorophyll content in winter wheat var. jinan no. 17. Moreover, calcium (Ca) levels and bioaccumulation of Hg in wheat leaves were studied with the technique of inductively coupled plasma sector field mass spectrometer (iCP-SF-MS). The study conducted a range of Hg concentrations from 0~500 mg Hg/kg in the dry weight soil. The soil was artificially contaminated with Hg as follows: 0, 100, 200, and 500 mg Hg/kg as HgCl 2 . at early stages of the wheat growth, both low and high concentration of Hg stimulates chlorophyll content, but inhibits chlorophyll content at later stages of the wheat growth. Furthermore, the concentrations of Ca and Hg in wheat leaves increased with the increasing concentration of Hg on the thirty-fourth day with the technique of iCP-SF-MS. The results indicate that Hg can accelerate the absorption of Ca in winter wheat and Hg stress may affect Ca levels in wheat leaves.
Spring stimulation feeding of honeybee colonies (Apis mellifera carpatica) is a very important technique for the encouragement of productive foraging. In addition to improving bee health by creating favorable conditions for the development of a beneficial intestinal bacterial flora, the use of prebiotic and probiotic supplements in the feed promotes good colony development, thus increasing the forager population. This paper presents the results for the economic benefit measured following use of supplements with acidifying substances (acetic and lactic acids) and probiotic products (Enterobiotics and Enterolactis Plus), as well as the synergism between them. A total of 110 bee colonies were studied, being fed with sugar syrup (1.4 L/colony weekly) between 25 March and 15 April, supplemented according to a controlled schedule. On 20 April the bees were transferred to a rapeseed field for foraging, and then at the beginning of May they were transferred elsewhere for acacia foraging. Honey production was measured for each experimental variant after each foraging period. Colonies fed with sugar syrup containing prebiotic and probiotic supplements registered higher honey production levels and showed a higher profit (ranging from +14.67% to +45.49%) in comparison with the control group.
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