pathogenic factor affecting bone fracture healing. Therefore, the effect of LPS on bone metabolism is relevant for bone healing. In this study, we investigated the effect of LPS on the expression of Toll-like receptor (TLR)-4 (an LPS receptor) by using real-time quantitative PCR and western blotting. We also examined the regulatory role of LPS in osteoblast differentiation by measuring the ALP activity, matrix mineralization, and ALP, OCN, and Runx2 mRNA (essential factors affecting osteoblast differentiation) expression in LPS-treated mouse osteoblast MC3T3-E1 cells. We also evaluated the effect of TLR-4 on LPS-mediated inhibition of osteoblast differentiation using RNA interference. LPS promotes TLR-4 mRNA and protein expression in MC3T3-E1 cells (P < 0.05, P < 0.01 or P < 0.001), and inhibits osteoblast differentiation by downregulating matrix mineralization and ALP activity (P < 0.05, P < 0.01 or P < 0.001), and suppressing the expression ALP, OCN, and Runx2 mRNA in MC3T3-E1 cells (P < 0.05 or P < 0.01). Conversely, RNAi-mediated TLR-4 knockdown abrogates the LPS-mediated inhibition of osteoblast differentiation (P < 0.05 or P < 0.01). In summary, LPS was shown to inhibit osteoblast differentiation by suppressing the expression of ALP, OCN, and Runx2 in a TLR-4-dependent manner. The results of this study may provide insights into the signal pathway of LPS-induced bone loss or delayed bone fracture healing.

Background: To provide the anatomic basis for the clinical application of the transpedicular screw fixation. Materials and methods: Thirty spine (C 2-L 5) specimens were used. The width of the pedicle cortex and width of the pedicle medullary cavity (WPC and WPMC), and the height of the pedicle cortex and height of the pedicle medullary cavity (HPC and HPMC) were measured at the isthmus of the pedicle using computed tomography (CT) scanning. Results: Width of the pedicle medullary cavity changed in a three-dovetailed-saddle shape with four peaks and three valleys, namely C 2 (high), C 4`5 (low), T 2 (high), T 4 (the lowest), T 12 (high), L 1 (low) and L 5 (the highest). HPMC of the cervical pedicle changed in a saddle shape, gradually increasing from C 5-L 5. WPC, WPMC, HPC and HPMC showed a regular change, respectively. In each segment, the superior border of the pedicle cortex had a nearly consistent thickness to the interior border within an identical pedicle, while the pedicle cortex thickness radio of the medial and lateral border was nearly 3:1 among the cervical pedicles, 2:1 among thoracic pedicles, and 1:1 among lumbar pedicles. Conclusions: Both HPMC and WPMC are the dominant factors for the choice of screw diameter, but HPMC should also be considered in C 2-T 1 pedicles, especially C 6 and C 7. Additionally, the screw for C 3-6 or T 4-6 pedicles should be about 3.0 mm in diameter.