This article analyzes the self-enterprising mechanism of research & development (R&D) engineers based on a case study of labor control in a high-tech company. The concept of “self as enterprise” refers to the form of labor control by allowing workers to self-manage according to the market principle. It is neither a form of normative control built upon accepting and internalizing market-oriented values nor responsible autonomy aiming to enhance workers' organizational commitment. R&D engineers' reproduction of current living standards depends heavily on performance-based salaries, which forces them to pragmatically comply with market rules. When both the reproduction of labor and the labor market have been overmarketized, R&D engineers are at high risk of downgrading their living standards and facing double job insecurity. Therefore, they must manage their work and life in an enterprising way to maximize labor value and prevent it from being devalued.

N6-methyladenosine (m6A) is the most prevalent RNA modification in eukaryotic mRNAs. Currently available detection methods for locus-specific m6A marks rely on RT-qPCR, radioactive methods, or high-throughput sequencing. Here, we develop a non-qPCR, ultrasensitive, isothermal, and naked-eye visible method for m6A detection based on rolling circle amplification (RCA) and loop-mediated isothermal amplification (LAMP), named m6A-Rol-LAMP, to verify putative m6A sites in transcripts obtained from the high-throughput data. When padlock probes hybridize to the potential m6A sites on targets, they are converted to circular form by DNA ligase in the absence of m6A modification, while m6A modification hinders the sealing of padlock probes. Subsequently, Bst DNA polymerase-mediated RCA and LAMP allow the amplification of the circular padlock probe to achieve the locus-specific detection of m6A. Following optimization and validation, m6A-Rol-LAMP can ultra-sensitively and quantitatively determine the existence of m6A modification on a specific target site as low as 100 amol under isothermal conditions. Detections of m6A can be performed on rRNA, mRNA, lincRNA, lncRNA and pre-miRNA from biological samples with naked-eye observations after dye incubation. Together, we provide a powerful tool for locus-specific detection of m6A, which can simply, quickly, sensitively, specifically, and visually determine putative m6A modification on RNA.

This article presents a qualitative empirical study of the impact of corruption and anti-corruption on the efficiency of China's bureaucratic system in developing a local economy. Drawing on 40 in-depth interviews and 98 days of participant observation, it first investigates the significance of extravagant position-related consumption in building personalized bureaucratic ties (patronage networks) and mobilizing resources for local economic development. It then examines the causal link between President Xi's campaign against corruption and extravagance and the rise of bureaucratic slack in local governments. The anti-extravagance campaign reduces the level of corruption in local government but it discourages local officials, who are motivated primarily by the desire to avoid risk and ensure political survival, from using banquets and gift-giving to build patronage networks, attract investment and mobilize development resources. The article concludes that corruption may contribute positively to the efficiency of a fragmented Chinese bureaucracy in fostering development at the local level, while the anti-corruption campaign compels local cadres to develop a new coping strategy – bureaucratic slack – for implementing policies and developing local economies.

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