The gut microbiota of German cockroaches may play a critical role in protecting cockroaches from fungal invasion and colonization. Removing certain bacteria from the B. germanica microbiota may facilitate microbial control using fungal pathogens. © 2017 Society of Chemical Industry.

The efficacy of a new and highly virulent Metarhizium anisopliae (Hypocreales: Clavicipitaceae) strain was evaluated against Blattella germanica (L.) (Blattaria: Blattellidae) in the laboratory; this strain was obtained and purified from field-collected Eupolyphaga sinensis cadavers. The status of this fungus as a new and genetically distinct species was supported by ITS sequence comparisons. The new strain was compared with other M. anisopliae isolates and was found to be highly infectious and virulent against B. germanica. The virulence of this new strain against different instars of male and female cockroaches at five conidia concentrations (1 × 105, 1 × 106, 1 × 107, 1 × 108, and 1 × 109 conidia/ml) was evaluated in vitro, and the mortality (measured as lethal concentrations, LC50) was determined. According to the pathogenicity test, M. anisopliae isolate EB0732 produced 100% mortality of one- to three-instar nymph (LC50 = 0.37 × 105conidia/ml) and 78.33% mortality of adult female (LC50 = 1.39 × 107 conidia/ml) at 15 d post-inoculation at a concentration of 1 × 109 conidia/ml. There was an overall significant effect on mortality between the age and sex of B. germanica. A M. anisopliae susceptibility test showed that the survival rate of cockroaches after treatment with topical applications and mixed infection was lower than per os. These studies shed light on a valuable integrated pest management against the German cockroach.