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BackgroundWound ointment (WO), a kind of Chinese medicine, can significantly promote fracture healing. The study aimed at analyzing the chemical composition and the effects of WO on fracture of rabbits and tried to explore the corresponding molecular mechanism in cytokine.MethodsThe qualitative and quantitative analysis of WO was conducted by liquid chromatography-mass spectrometry (LC–MS). Fifty-four Zealand mature male rabbits were randomly divided into 3 groups: Control group, Yunnan Baiyao (YB) group and WO group. All the rabbits suffered a fracture of right radius and were then stabilized with an external fixator. Treated with different methods, fracture healing was observed. The bone specimens were subjected to radiograph, immunohistochemistry (IHC) analysis, hematoxylin–eosin staining (HE), western blot and enzyme linked immunosorbent assay (ELISA).ResultsA total of 12 active compositions were detected by LC–MS. Radiographs showed a considerably better bone healing and remodeling of the fracture in WO group. HE experiments showed that a large number of osteoclasts appeared in the early stage when treated with WO. In immunohistochemistry (IHC), western blot and ELISA test, significant increases in vascular endothelial growth factor (VEGF) expression were observed in WO group compared with other two groups.ConclusionsWound ointment contained active compositions which efficiently promoted fracture healing through increasing the expression of VEGF. Trial Registration Not applicableElectronic supplementary materialThe online version of this article (doi:10.1186/s13020-017-0152-y) contains supplementary material, which is available to authorized users.

The water absorption of ultra‐high molecular weight polyethylene (UHMWPE) and polyformaldehyde (POM) in different hydrostatic pressure has been investigated by means of high pressure immerse experiments and molecular dynamics simulation. Experiment results showed that water absorption of UHMWPE and POM tended to decrease with the rise of environmental pressure of water within 0 ~ 3 MPa. Water absorption of POM was far greater than UHMWPE under all kinds of hydraulic pressure. When the pressure is 3 MPa, the water absorption of POM is reduced by about 15.2% compared with that under normal pressure, while that of UHMWPE is reduced by about 41.64%. Simulation results showed that water molecules were bonded with UHMWPE molecular chain by means of Van der Waals force, and water molecules were bonded with POM molecular chain by means of Van der Waals force and hydrogen bonds, which provided potent theoretical support for the water absorption properties of UHMWPE and POM.