Based on requirement of intensity and thickness for the tubular nonwoven composite material by the big pipeline rehabilitation, the preparation and properties of the tubular nonwoven composite material were researched. To add fabric to reinforce the tubular nonwoven composite material; two pieces or more of the reinforced nonwoven were designed, so the structure of the tubular nonwoven composite material with the seams in circle direction equidistant, the structure of the tubular nonwoven composite material with many seams is symmetry, during inversion, the collecting of the stress of the single seam can be avoided, so the crazing of the seam is prevented; when the reinforced nonwoven is coverd film, at its two sides the areas for sewing were obligated, the difficulty of the sewing is decreased, the efficiency of the sewing is increased, and the thickness of the seam is decreased, the film jointing of the seam become more easy, the airproof of the tubular nonwoven composite material can be guaranteed. The test result shows that mechanical property, width and airproof property can meet the design requirement, and the corrosion resistance can meet the requirement of the standard.

Restoring the normal structure and function of injured tendons is one of the biggest challenges faced by the Department of Orthopedics and Sports Medicine. Tendon-derived stem cells (TDSCs), a new type of pluripotent stem cells with multidirectional differentiation potential, are expected to be promising cell seeds for the treatment of tendon injury and tendon-bone healing in the future. In this study, tendon stem cells were successfully isolated from human tissues, which were positive for markers CD44, CD90, and CD105, and exhibited clonality and multilineage differentiation ability. Analysis of single-cell sequencing results and mass spectrometry identification results showed that there were differences in protein expression during CTGF-induced TDSC tendon differentiation. Reverse Co-IP, qPCR, WB, and immunofluorescence detection all confirmed that CTGF directly interacts with KIT, thereby mediating the transcription factor HES1 to regulate the Wnt/β-catenin signaling pathway (GSK3β, β-catenin, TCF4). ChIP-qPCR and dual-luciferase reporter gene assays indicated that HES1 regulates stem cell differentiation by directly regulating the expression of GSK3β in the Wnt/β-catenin pathway. Rats were treated with TDSCs overexpressing the KIT gene after repair surgery. This method had a more ideal recovery effect than other methods through animal behavioral scores, mechanical properties testing, and HE staining tissue observation. This study found that the use of modified human tendon stem cells (hTDSC) could promote graft ligamentization and tendon-bone healing after ACL reconstruction, which could provide an effective way for faster and better recovery from tendon injury.

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