In the present study, we constructed the recombinant plasmid IL10-PEGFP-C1 and successfully transfected into human mesenchymal stem cells. After culturing for 72 h, the levels of IL6 and TNF-α in the supernatant of the MSCs-IL10 group were significantly lower than the vector group and the control group (17.6 ± 0.68vs73.8 ± 0.8 and 74.4 ± 1.5) µg/L and (65.05 ± 3.8 vs 203.2 ± 2.4 and 201.3 ± 3.7) µg/L, respectively (p < 0.001) .The animal experiments showed that the volume of subcutaneous tumors in the MSCs-IL10 group in vivo was a significantly less level compared to that in MSC control and the blank control groups (76.84 ± 20.11) mm 3 vs (518. 344 ± 48.66) mm 3 , (576.99± 49.88) mm 3 , (P < 0. 05) and they have a longer life time. Further we found the mass concentrations of IL6 and TNF-α in the blood serum of MSC-IL10 group were lower than the vector group and the control group (64.42 ± 10.9 vs120.83 ± 15.52 and 122.65 ± 13.71) and (40.05 ± 5.63 vs 126.78 ±1.89 and 105.83 ± 2.16) µg/L respectively (p < 0.001). CD31 immunohistochemistry and alginate encapsulation experiments showed tumor angiogenesis were inhibited in MSCs-IL10 group in comparison to the control and vector group (P < 0.001), FITC-labeled dextran intake was also lower than the other groups (P < 0.01). Collectively, this study suggested IL10 could inhibit the growth of the transplanted tumor in vivo and prolong survival of mice, and the primary mechanism may be the indirect inhibition of pro-inflammatory cytokines IL6 and TNF-α secretion and tumor angiogenesis formation.

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Narrow band internet of things (NB-IoT), as an important low-power wide-area coverage of 5G with independent intellectual property rights, has great potential in small data monitoring of urban power transmission and distribution. However, it is extremely easy to cause congestion and overloading of the system upon large-scaled terminal requests for random access, which will decrease network access performance and bring great challenges to promote NB-IoT in urban power Internet of Things. On this basis, a mathematical model of network load was constructed to estimate and predict load changes. Then, a dynamic adjustment functional model was set up according to dynamic adjustment of access parameter optimization based on loads. Based on above idea, a random access algorithm based on dynamic parameter adjustment in accordance to dynamic priority was developed. It increases the success rate of devices access to NB-IoT system, decreases access delay of devices, and relieves network congestion. According to simulation results, the proposed algorithm has higher access success rate, lower total delay. And it guarantees delay for important node users and more optimized network performances compared with traditional network access algorithms.

The application of Low-Power Wide-Area Network (LPWAN) technology in wireless monitoring of power terminal equipment is studied in this paper. From the aspects of hardware and software design of monitoring terminal, communication protocol, remote data management cloud platform, the wireless monitoring system of power terminal based on Narrow Band Internet of Things (NB-IoT) is designed in detail. STM32L151 is chosen as the main controller of the monitoring terminal owing to its low power consumption. After simple edge calculation and data packaging, the terminal equipment status information is reported to the remote data management platform via NB-IoT network based on system setting. The platform supports various functions such as a variety of terminal access methods, terminal identity authentication, parameter configuration, data forwarding, and terminal abnormal data information reporting and so on. In order to improve the User Datagram Protocol (UDP) transmission reliability, the terminal drop detection mechanism is designed on UDP communication protocol. Eventually, the system is applied in monitoring power terminal equipment status such as transmission and distribution pole tower, power well cover, cable trench cover, knife brake of distribution line, etc. The experimental results show that the power terminal monitoring system based on NB-IoT can achieve unified access and centralized management of power equipment status information in different power scenarios, and this system greatly enhances the reliability and efficiency of power system terminal equipment operation and maintenance, and has value in engineering.

Background The F11 receptor belongs to the immunoglobulin superfamily and is expressed in epithelial and endothelial cells. F11R mediates the formation of tight junctions between the epithelium and endothelium, and participates in the invasion and metastasis of tumor cells. We have previously shown that the F11R gene is closely related to KRas (P= 0.76), a known therapeutic target for pancreatic cancer (PCa). In recent years, it has been found that F11R is expressed in different tumors and has biological effects.However, according to different tumor cases, different cell lines and experimental conditions, the regulatory results and mechanisms of F11R on tumor are different, even contradictory,and the expression, clinical significance and biological mechanism of F11R in tumor tissues have not been reported in detail. Results To investigate the role of F11R in carcinogenesis of PCa and the potential of F11R as a therapies target for PCa, we silenced F11R (-/-) in the PCa cell line PANC-1 (known to express high levels of KRas) using lentiviral approaches.We found that F11R silencing led to decreased cell proliferation, a loss of cell invasiveness, reduced colony forming ability, cell cycle arrest in G1 phase, cells apoptosis enhanced, and ros enhanced. In vitro data showed that inhibition of F11R decreased proliferation and invasiveness of cancer cells.The present results suggest that F11R may be a promising therapeutic target for PCa. Conclusions This study used bioinformatics combined with gene chip data to find the gene F11R, which is closely related to KRAS gene, and we used lentivirus to package shRNA plasmid to interfere with the gene F11R in pancreatic cancer panc-1 cells. A series of biobehavioral studies indicated the biobehavioral function and malignancy of panc-1 in pancreatic cancer cells with negative regulation of F11R gene.Based on this, we need to continue to clarify the expression of F11R gene in clinical case samples to determine whether F11R gene can be a new therapeutic target for pancreatic cancer.