Background Protein arginine methylation is a prevalent post-translational modification. The protein arginine methyltransferase family (PRMT) is involved in many cellular processes in eukaryotes, including transcriptional regulation, epigenetic regulation, RNA metabolism, and DNA damage repair. Toxoplasma gondii , an opportunistic protozoan parasite, encodes five conserved PRMTs. PRMT5 is thought to be responsible for substantial PRMT activity in T. gondii ; however, it has not yet been characterized. Methods We tagged the 3′ end of the endogenous TgPRMT5 genomic locus with sequence encoding a 3X hemagglutinin (HA) epitope. IFA and WB were performed to check the expression and subcellular localization of TgPRMT5 in tachyzoites and bradyzoites. In vitro methylation assays were performed to determine whether endogenous TgPRMT5 has arginine methyltransferase activity. Results IFA and WB results showed that T. gondii PRMT5 (TgPRMT5) was localized in the cytoplasm in the tachyzoite stage; however, it shifts largely to the nuclear compartment in the bradyzoite stage. The in vitro methylation showed that TgPRMT5 has authentic type II PRMT activity and forms monomethylarginines and symmetric dimethylarginines. Conclusions We determined the expression and cellular localization of TgPRMT5 in tachyzoites and bradyzoites and confirmed its type II PRMT activity. We demonstrated the major changes in expression and cellular localization of TgPRMT5 during the tachyzoite and bradyzoite stages in T. gondii . Our findings suggest that TgPRMT5 protein may be involved in tachyzoite-bradyzoite transformation.

As a new FACTS device, thyristor controlled braking resistor (TCBR) is one of the effective devices to enhance transient stability of generator. In this paper, a coordinated controller for TCBR and hydro-generator excitation system with the multi-index nonlinear coordinate control method based differential geometry theory is proposed. By means of Hartman-Grobman theorem, differential geometry multi-index nonlinear control (DGMINC) design method can reassign the closed-loop system eigenvalues of linear approximate system to the nonlinear system via appropriately selecting output function parameter matrix. Therefore, the system can get good control performance. The simulation results show that TCBR and generator excitation system controlled by this coordinate control strategy can decrease the generator acceleration area rapidly when the severe fault occurred and increase deceleration area significantly after the fault is cleared. So that, power system transient stability limitation improved significantly.

To find out an alternative of coal saving, a kind of microalgae, Chlorella vulgaris (C. vulgaris) which is widespread in fresh water was studied by digital blast drying system. The effect of the moisture content, drying thickness and temperature on the drying process of C. vulgaris were investigated. The results indicated that when the drying temperature is high, the moisture content is low and the material thickness is small, the drying time is short. The drying process of C.vulgaris can be divided into two stages, and the mass loss is mainly occurred in the second stage . The results will provide guidance for design of drying process and dryer of microalgae.

The permanent magnet synchronous motor take place under certain conditions chaotic phenomenon, according to the differential geometry theory, using the method of linear completely accurate, to design the control chaotic control law, and then through the experiments to verify its validity.