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BACKGROUND: The authors prospectively evaluated the correlation between technetium‐99m methoxyisobutyl isonitrile (99mTc‐MIBI) accumulation in tumors and response to induction chemotherapy in patients with nasopharyngeal carcinoma (NPC). METHODS: Eighty‐six patients with locally advanced NPC underwent single‐photon emission computed tomography 15 minutes after an intravenous injection of 740 megabecquerels (20 mCi) 99mTc‐MIBI before chemotherapy. The tumor uptake ratio (TUR) was calculated. Two weeks after the second cycle of combined chemotherapy with 5‐fluorouracil (5‐FU) and cisplatin (DDP), the tumor response rate was evaluated. The correlation between 99mTc‐MIBI accumulation in tumors and response to chemotherapy with 5‐FU/DDP was examined. RESULTS: Positive accumulation of 99mTc‐MIBI in tumors was observed in 76 patients (88.4%). The tumor response was a complete response (CR) in 8 patients, a partial response (PR) in 68 patients, stable disease (SD) in 9 patients, and progressive disease (PD) in 1 patient. The response rate (CR and PR) to 5‐FU/DDP chemotherapy in patients who had positive 99mTc‐MIBI accumulation (tumor uptake ratio [TUR] >1.1) was higher than that in patients who had negative 99mTc‐MIBI accumulation (TUR ≤1.1; 98.7% vs 10%; P < .001). CONCLUSIONS: Patients with negative 99mTc‐MIBI accumulation were resistant to 5‐FU/DDP chemotherapy. 99mTc‐MIBI imaging in patients with NPC was capable of predicting tumor response to chemotherapy with 5‐FU/DDP and can help in the selection of patients for induction chemotherapy. Cancer 2011. © 2010 American Cancer Society.

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Fermentative hydrolysate of extruded corn gluten with higher solubility and antioxidative property by Bacillus natto was prepared. Extrusion of corn gluten was applied as pretreatment before fermentation. The best fermentative hydrolysis results can be obtained by fermenting at 34°C for cultivation time of 32 h, and flask rotation speed and initial pH of culture media were 200 r/min and 6.5-7.0 respectively. Soluble protein content and antioxidative activity of the fermentative hydrolysate reached 24.95 mg/ml and 259.21 U/ml respectively under the optimized culture condition. The molecular weight of the peptides in the optimal hydrolysate distributed mainly over 860-5300 Da determined by gel filtration chromatography. The hydrolysates displayed good solubility and antioxidative activity.

A strong fibrin-specific fibrinolytic enzyme was produced by liquid culture ofCoprinus comatusYY-20. Soybean cake powder and sucrose as substrates resulted in the highest fibrinolytic activity. The optimal temperature for the production of the enzyme was found to be 21-25°C in the optimal medium (Soybean cake powder 50 g/L, sucrose 20 g/L). The optimal initial pH of the medium was found to be at the range of 5-8. The maximum fibrinolytic activity was observed at the stationary phase of cell growth. These results suggest that the culture liquid ofCoprinus comatusYY-20 has potential as a source of physiologically active fibrinolytic enzymes.