Abstract. We have isolated a cDNA clone (mERD2) for the mammalian (bovine) homologue of the yeast ERD2 gene, which codes for the yeast HDEL receptor. The deduced amino acid sequence bears extensive homology to its yeast counterpart and is almost identical to a previously described human sequence. The sequence predicts a very hydrophobic protein with multiple membrane spanning domains, as confirmed by analysis of the in vitro translation product. The protein encoded by mERD2 (p23) has widespread occurrence, being present in all the cell types examined. p23 was localized to the cis-side of the Golgi apparams and to a spotty intermediate compartment which mediates ER to Golgi transport. A majority of the intracellular staining could be accumulated in the intermediate compartment by a low temperature (15~ or brefeldin A. During recovery from these treatments, the spotty intermediate compartment staining of p23 was shifted to the perinuclear staining of the Golgi apparatus and tubular structures marked by p23 were observed. These tubular structures may serve to mediate transport between the intermediate compartment and the Golgi apparatus.
The numerical simulation was conducted to analyse the fatigue crack growth in gear with the finite element codes ansys (ANSYS, Inc. Canonsburg, Pennsylvania, USA.) and franc3d (Fracture Analysis Consultants, Inc. Ithaca, New York, USA.), and the corresponding fatigue test was also carried out. During the simulation, the location of maximal stress induced by the external force was first determined by the code ansys, and then the obtained results were imported into the franc3d to analyse the crack growth. The analysed results were input into the codes ansys and franc3d again to compute the stress and the stress intensity factor in the following steps. After several rounds of analysis, the results of the fatigue crack propagation were obtained. The investigations show that the crack mode I is dominant during the crack growth and the stress intensity factor KI raises with increase of crack growth length and a series of quarter‐elliptical cross sections of the ruptured gear tooth are obtained. The simulation results are in good accordance with experimental findings.
Tb (AUFA)32H2O, a rare earth terbium complex,was synthesized by introducing 4-(11-azobenzene-undecyloxy) ferrocene acid (AUFA) as the ligand. This complex was characterized by elemental analysis, MS, IR, Raman, UV spectroscopy and fluorescence spectrophotometry. The complex exhibited ligand-sensitized green emission, and Tb (AUFA)32H2O had a higher sensitized luminescence efficiency and a longer lifetime than the other terbium complexes (DPC: 2, 6-Pyridinedicarboxylic acid, Aspirin: 2-ethanoylhydroxybenzoic acid). The organic-inorganic thin film of complexe Tb (AUFA)32H2O in nanoTiO2 was fabricated, and the nanoTiO2 has been used in the luminescence layer to change the luminescence property of complexes Tb (AUFA)32H2O. It was found that there was an efficient energy transfer process between the ligands and metal ions. Moreover, In an ITO/PVK/Tb (AUFA)32H2O/Al device, Tb3+ may be excited by intramolecular energy transfer from the ligand, as observed by electroluminescence. The main emitting peak at 545 nm can be attributed to the 5D4→7F5 transition of the Tb3+ ion, and this process results in the enhancement of the green emission from the electroluminescence device.
In this paper, a mode conversion model is proposed to increase the capacity of optical fiber communication systems. In this model, a spatial spectral matching method is used to convert the original mode to the desired mode for mode division multiplexing in optical fibers. A binary phase spatial light modulator is employed on the Fourier plane as a spatial filter. Numerical results show that the original modes can be converted to the desired modes.
Slewing bearings are the basic structural components widely used in construction machinery industry. Their stress status and distribution have great influence on the service life. A finite element approach for a single-row slewing bearing is established based on the bilinear isotropic model. The stress status at different loading conditions is simulated with the bearing geometric and material parameters. The result shows that the numerical simulation is reasonable in comparison with the analytical result obtained by Hertz’s theory and provides reliable design guideline for slewing bearings.
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